Table 1 Composition of central and... | American Society of Hematology

Table 1

Composition of central and peripheral lymphatic organs

Cell populations	WT	Bmf^−/−	Bim^−/−	DKO
Thymus*
Total (×10⁷)	23.28 ± 2.95	31.61 ± 0.90	28.95 ± 5.10	24.39 ± 2.46
CD4⁻8^-	0.71 ± 0.02	0.95 ± 0.10†	2.85 ± 0.66†‡	2.36 ± 0.23†‡
CD4⁺8⁺	19.71 ± 3.78	27.5 ± 0.77	18.5 ± 2.98	16.34 ± 1.92‡
CD4⁺8^-	2.17 ± 0.29	2.20 ± 0.08	5.58 ± 1.15†‡	3.96 ± 0.46†‡
CD4⁻8⁺	0.70 ± 0.10	0.92 ± 0.06	1.54 ± 0.20†‡	1.74 ± 0.23†‡
Spleen
Total (×10⁷)	13.89 ± 0.87	21.21 ± 2.52†	27.9 ± 4.64†	41.64 ± 4.14†‡§
B cells
B220⁺	8.12 ± 0.88	14.5 ± 1.98†	14.91 ± 2.21†	26.45 ± 3.48†‡§
T1	0.95 ± 0.23	1.44 ± 0.3	1.85 ± 0.44	3.31 ± 0.58†‡
T2	0.93 ± 0.07	1.88 ± 0.3†	1.98 ± 0.4†	3.58 ± 0.38†‡§
FO	5.19 ± 0.59	10.4 ± 1.54†	9.87 ± 1.56†	15.09 ± 1.76†§
MZ	1.08 ± 0.16	1.53 ± 0.28	1.39 ± 0.25	2.35 ± 0.49†
PC/PB	1.37 ± 0.19	2.24 ± 0.35†	3.97 ± 0.6†	8.4 ± 1.24†‡§
T cells
TCRβ⁺	3.98 ± 0.39	4.62 ± 0.41	7.88 ± 1.39†	8.04 ± 0.78†‡
Naïve CD4	1.42 ± 0.19	1.48 ± 0.09	1.84 ± 0.49	1.38 ± 0.2
T_CM CD4	0.51 ± 0.11	0.56 ± 0.13	1.12 ± 0.32	1.14 ± 0.26
T_EM CD4	0.41 ± 0.03	0.63 ± 0.12	1.05 ± 0.22†	1.06 ± 0.18†
Naïve CD8	0.78 ± 0.13	0.87 ± 0.18	1.3 ± 0.36	1.34 ± 0.2
T_CM CD8	0.46 ± 0.06	0.51 ± 0.11	1.01 ± 0.18†	1.13 ± 0.18†‡
T_EM CD8	0.24 ± 0.06	0.39 ± 0.15	0.63 ± 0.12†	0.86 ± 0.19†
Ratio CD4/CD8	1.66 ± 0.08	1.54 ± 0.09	1.39 ± 0.08†	1.13 ± 0.06†‡§
Ratio B220/TCRβ	2.06 ± 0.16	3.12 ± 0.25†	1.96 ± 0.1‡	3.27 ± 0.22†§
Myeloid cells
Granulocytes	0.087 ± 0.02	0.16 ± 0.06	0.08 ± 0.03	0.12 ± 0.01
Monocytes	0.28 ± 0.1	0.4 ± 0.15	0.53 ± 0.17	0.56 ± 0.21
Ter119⁺	0.99 ± 0.15	2.46 ± 0.56†	4.07 ± 1.05†	5.43 ± 1.07†‡
Bone marrow
Total (×10⁶)	33.45 ± 4.76	36.44 ± 6.77	29.45 ± 4.71	35.03 ± 4.06
B cells
B220⁺	8.7 ± 1.28	9.87 ± 1.46	10.06 ± 1.59	14.49 ± 1.73†
Pro-B cells	0.71 ± 0.1	0.73 ± 0.14	0.92 ± 0.19	1.02 ± 0.08†
Pre-B cells	4.62 ± 0.61	5.74 ± 1.13	4.27 ± 0.85	5.08 ± 0.94
IM B cells	2.41 ± 0.58	2.52 ± 0.42	2.7 ± 0.51	3.63 ± 0.77
M B cells	2.15 ± 0.31	3.21 ± 0.79	4 ± 0.73†	7.63 ± 1.08†‡§
Percentage small pre-B	65.14 ± 4.35	67.88 ± 1.93	71.65 ± 2.45	80.07 ± 3.87†‡
Myeloid cells
Granulocytes	6.17 ± 0.77	6.76 ± 0.99	4.17 ± 0.66	3.65 ± 0.62†‡
Monocytes	3.36 ± 0.45	3.44 ± 0.54	3 ± 0.48	3.03 ± 0.85
Ter119⁺	6.96 ± 1.56	9.56 ± 2.82	6.32 ± 1.71	7.05 ± 1.63
PBL
WBC/μL¶	7116 ± 808	15 180 ± 2 446†	27 557 ± 4 016†‡	33 725 ± 4 938†‡
B220⁺	4867 ± 796	11 959 ± 2 020†	21 207 ± 3 543†‡	25 577 ± 4 358†‡
CD4⁺	742 ± 248	920 ± 263	1 808 ± 573	1 338 ± 365
CD8⁺	564 ± 145	908 ± 221	2 308 ± 606†	1 780 ± 307†
Monocytes	564 ± 166	855 ± 180	1 201 ± 354	2 494 ± 1007
Granulocytes	621 ± 108	684 ± 139	709 ± 123	1 784 ± 383†§

Cell populations	WT	Bmf^−/−	Bim^−/−	DKO
Thymus*
Total (×10⁷)	23.28 ± 2.95	31.61 ± 0.90	28.95 ± 5.10	24.39 ± 2.46
CD4⁻8^-	0.71 ± 0.02	0.95 ± 0.10†	2.85 ± 0.66†‡	2.36 ± 0.23†‡
CD4⁺8⁺	19.71 ± 3.78	27.5 ± 0.77	18.5 ± 2.98	16.34 ± 1.92‡
CD4⁺8^-	2.17 ± 0.29	2.20 ± 0.08	5.58 ± 1.15†‡	3.96 ± 0.46†‡
CD4⁻8⁺	0.70 ± 0.10	0.92 ± 0.06	1.54 ± 0.20†‡	1.74 ± 0.23†‡
Spleen
Total (×10⁷)	13.89 ± 0.87	21.21 ± 2.52†	27.9 ± 4.64†	41.64 ± 4.14†‡§
B cells
B220⁺	8.12 ± 0.88	14.5 ± 1.98†	14.91 ± 2.21†	26.45 ± 3.48†‡§
T1	0.95 ± 0.23	1.44 ± 0.3	1.85 ± 0.44	3.31 ± 0.58†‡
T2	0.93 ± 0.07	1.88 ± 0.3†	1.98 ± 0.4†	3.58 ± 0.38†‡§
FO	5.19 ± 0.59	10.4 ± 1.54†	9.87 ± 1.56†	15.09 ± 1.76†§
MZ	1.08 ± 0.16	1.53 ± 0.28	1.39 ± 0.25	2.35 ± 0.49†
PC/PB	1.37 ± 0.19	2.24 ± 0.35†	3.97 ± 0.6†	8.4 ± 1.24†‡§
T cells
TCRβ⁺	3.98 ± 0.39	4.62 ± 0.41	7.88 ± 1.39†	8.04 ± 0.78†‡
Naïve CD4	1.42 ± 0.19	1.48 ± 0.09	1.84 ± 0.49	1.38 ± 0.2
T_CM CD4	0.51 ± 0.11	0.56 ± 0.13	1.12 ± 0.32	1.14 ± 0.26
T_EM CD4	0.41 ± 0.03	0.63 ± 0.12	1.05 ± 0.22†	1.06 ± 0.18†
Naïve CD8	0.78 ± 0.13	0.87 ± 0.18	1.3 ± 0.36	1.34 ± 0.2
T_CM CD8	0.46 ± 0.06	0.51 ± 0.11	1.01 ± 0.18†	1.13 ± 0.18†‡
T_EM CD8	0.24 ± 0.06	0.39 ± 0.15	0.63 ± 0.12†	0.86 ± 0.19†
Ratio CD4/CD8	1.66 ± 0.08	1.54 ± 0.09	1.39 ± 0.08†	1.13 ± 0.06†‡§
Ratio B220/TCRβ	2.06 ± 0.16	3.12 ± 0.25†	1.96 ± 0.1‡	3.27 ± 0.22†§
Myeloid cells
Granulocytes	0.087 ± 0.02	0.16 ± 0.06	0.08 ± 0.03	0.12 ± 0.01
Monocytes	0.28 ± 0.1	0.4 ± 0.15	0.53 ± 0.17	0.56 ± 0.21
Ter119⁺	0.99 ± 0.15	2.46 ± 0.56†	4.07 ± 1.05†	5.43 ± 1.07†‡
Bone marrow
Total (×10⁶)	33.45 ± 4.76	36.44 ± 6.77	29.45 ± 4.71	35.03 ± 4.06
B cells
B220⁺	8.7 ± 1.28	9.87 ± 1.46	10.06 ± 1.59	14.49 ± 1.73†
Pro-B cells	0.71 ± 0.1	0.73 ± 0.14	0.92 ± 0.19	1.02 ± 0.08†
Pre-B cells	4.62 ± 0.61	5.74 ± 1.13	4.27 ± 0.85	5.08 ± 0.94
IM B cells	2.41 ± 0.58	2.52 ± 0.42	2.7 ± 0.51	3.63 ± 0.77
M B cells	2.15 ± 0.31	3.21 ± 0.79	4 ± 0.73†	7.63 ± 1.08†‡§
Percentage small pre-B	65.14 ± 4.35	67.88 ± 1.93	71.65 ± 2.45	80.07 ± 3.87†‡
Myeloid cells
Granulocytes	6.17 ± 0.77	6.76 ± 0.99	4.17 ± 0.66	3.65 ± 0.62†‡
Monocytes	3.36 ± 0.45	3.44 ± 0.54	3 ± 0.48	3.03 ± 0.85
Ter119⁺	6.96 ± 1.56	9.56 ± 2.82	6.32 ± 1.71	7.05 ± 1.63
PBL
WBC/μL¶	7116 ± 808	15 180 ± 2 446†	27 557 ± 4 016†‡	33 725 ± 4 938†‡
B220⁺	4867 ± 796	11 959 ± 2 020†	21 207 ± 3 543†‡	25 577 ± 4 358†‡
CD4⁺	742 ± 248	920 ± 263	1 808 ± 573	1 338 ± 365
CD8⁺	564 ± 145	908 ± 221	2 308 ± 606†	1 780 ± 307†
Monocytes	564 ± 166	855 ± 180	1 201 ± 354	2 494 ± 1007
Granulocytes	621 ± 108	684 ± 139	709 ± 123	1 784 ± 383†§

Total numbers of hemopoietic cells from 6- to 8-week-old animals were calculated by counting single-cell suspensions derived from the indicated tissues. Cells were subsequently analyzed by flow cytometry after staining for cell surface markers: T1 B cells (B220⁺sIgM^highCD21^low), T2 B cells (B220⁺sIgM^highCD21^high), FO B cells (B220⁺CD21⁺CD23⁺), MZ B cells (B220⁺CD21⁺CD23⁻), PC/PBs (B220^+/lowCD138⁺), naïve CD4⁺ or CD8⁺ T cells (CD62L^highCD44^low), effector/memory (T_EM) CD4⁺ or CD8⁺ T cells (CD62L^lowCD44^high), central memory (T_CM) CD4⁺ or CD8⁺ T cells (CD62L^highCD44^high), granulocytes (Gr-1⁺CD11b⁺), monocytes (Gr-1⁻CD11b⁺), pro-B cells (B220^lowIgM⁻CD43⁺), pre-B cells (B220^lowIgM⁻CD43⁻), immature (IM) B cells (B220^lowIgM⁺), and mature (M) B cells (B220^highIgM⁺). Numbers represent the mean ± standard error of the mean (SEM) of 5 to 8 mice per genotype. Total cellularity of both femora is presented.

Only female animals were analyzed.

†

P < .05 compared with WT mice.

‡

P < .05 compared with Bmf^−/− mice.

P < .05 compared with Bim^−/− mice.

Cell number assessed in a Vet-ABC blood sample analyzer.

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