Table 1

Saturation and competition binding of unconjugated anti-CD33 antibodies and SGN-CD33A on human CD33+ cell lines

AntibodySaturation binding, nMCompetition binding, nM
HL-60HEL 92.1.7293F-huCD33
m2H12 NT NT 
h2H12 NT NT 
h2H12ec 1.3 0.6 5.6 
SGN-CD33A 1.2 0.5 NT 
AntibodySaturation binding, nMCompetition binding, nM
HL-60HEL 92.1.7293F-huCD33
m2H12 NT NT 
h2H12 NT NT 
h2H12ec 1.3 0.6 5.6 
SGN-CD33A 1.2 0.5 NT 

Saturation binding studies on HL-60 and HEL 92.1.7 cell lines were performed in the presence of 10% human serum. Detection was done with anti-human κ light chain FITC-labeled secondary antibody. Competition binding studies were performed with 6 nM AF488-labeled m2H12 antibody on 293F cells stably expressing 200 000 copies of full-length human CD33. NT, not tested.

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