Saturation and competition binding of unconjugated anti-CD33 antibodies and SGN-CD33A on human CD33+ cell lines
| Antibody . | Saturation binding, nM . | Competition binding, nM . | |
|---|---|---|---|
| HL-60 . | HEL 92.1.7 . | 293F-huCD33 . | |
| m2H12 | NT | NT | 7 |
| h2H12 | NT | NT | 5 |
| h2H12ec | 1.3 | 0.6 | 5.6 |
| SGN-CD33A | 1.2 | 0.5 | NT |
| Antibody . | Saturation binding, nM . | Competition binding, nM . | |
|---|---|---|---|
| HL-60 . | HEL 92.1.7 . | 293F-huCD33 . | |
| m2H12 | NT | NT | 7 |
| h2H12 | NT | NT | 5 |
| h2H12ec | 1.3 | 0.6 | 5.6 |
| SGN-CD33A | 1.2 | 0.5 | NT |
Saturation binding studies on HL-60 and HEL 92.1.7 cell lines were performed in the presence of 10% human serum. Detection was done with anti-human κ light chain FITC-labeled secondary antibody. Competition binding studies were performed with 6 nM AF488-labeled m2H12 antibody on 293F cells stably expressing 200 000 copies of full-length human CD33. NT, not tested.