IC50 values determined by SPR-based and tenase activity assays
| Compound ID . | ChemBridge ID . | IC50 for inhibition of FVIII-membrane binding, µM (mean) . | IC50 for inhibition of tenase activity, µM (mean) . | IC50 for inhibition of C2 domain-membrane binding, µM (mean) . |
|---|---|---|---|---|
| A11-8* | 5992471 | 3.3 | 9.2 | 3.9 |
| D7-5 | 5651006 | 2.1 | 26.9 | 2.4 |
| H5-8 | 6844287 | 2.7 | 3.5 | 47 |
| C11-10 | 7687521 | 3.5 | 4.2 | 4.6 |
| C8-4 | 5898659 | 9.6 | 5.6 | 52.5 |
| D11-5 | 5941257 | 6.7 | 10.1 | 22.9 |
| D6-7 | 6656941 | 19.9 | 59.7 | 33.7 |
| C8-5 | 5740628 | 10.6 | 18.5 | 33.6 |
| B7-5 | 5731791 | 8.1 | 38.6 | 20.2 |
| Compound ID . | ChemBridge ID . | IC50 for inhibition of FVIII-membrane binding, µM (mean) . | IC50 for inhibition of tenase activity, µM (mean) . | IC50 for inhibition of C2 domain-membrane binding, µM (mean) . |
|---|---|---|---|---|
| A11-8* | 5992471 | 3.3 | 9.2 | 3.9 |
| D7-5 | 5651006 | 2.1 | 26.9 | 2.4 |
| H5-8 | 6844287 | 2.7 | 3.5 | 47 |
| C11-10 | 7687521 | 3.5 | 4.2 | 4.6 |
| C8-4 | 5898659 | 9.6 | 5.6 | 52.5 |
| D11-5 | 5941257 | 6.7 | 10.1 | 22.9 |
| D6-7 | 6656941 | 19.9 | 59.7 | 33.7 |
| C8-5 | 5740628 | 10.6 | 18.5 | 33.6 |
| B7-5 | 5731791 | 8.1 | 38.6 | 20.2 |
All values are averages of at least 2 dose-response curves (for SPR-based methods) and of triplicates (tenase activity assay).