Results of AS-PCR in HCL cases and other B-cell neoplasms
| Sample type . | Leukemic cells . | No. of cases . | Mutated cases, no. (%) . |
|---|---|---|---|
| Hairy cell leukemia | |||
| Blood, pretreatment | 1%-90% | 81 | 81 (100) |
| Blood, MRD | 0.1%-13% | 11 | 11 (100) |
| Blood, complete remission | Not detectable | 16 | 0 (0) |
| BM biopsies | 30%-80% | 14 | 14 (100) |
| Purified leukemic cells | ≥90% | 17 | 17 (100) |
| Splenic marginal zone lymphoma | 2%-97% | 61 | 0 (0) |
| Splenic lymphoma/leukemia unclassifiable | 15%-97% | 18* | 0 (0) |
| Chronic lymphocytic leukemia | 18%-98% | 31 | 0 (0) |
| CD5-negative mature B-cell neoplasm unclassifiable | 15%-97% | 5 | 0 (0) |
| Healthy blood donors | Not present | 9 | 0 (0) |
| Sample type . | Leukemic cells . | No. of cases . | Mutated cases, no. (%) . |
|---|---|---|---|
| Hairy cell leukemia | |||
| Blood, pretreatment | 1%-90% | 81 | 81 (100) |
| Blood, MRD | 0.1%-13% | 11 | 11 (100) |
| Blood, complete remission | Not detectable | 16 | 0 (0) |
| BM biopsies | 30%-80% | 14 | 14 (100) |
| Purified leukemic cells | ≥90% | 17 | 17 (100) |
| Splenic marginal zone lymphoma | 2%-97% | 61 | 0 (0) |
| Splenic lymphoma/leukemia unclassifiable | 15%-97% | 18* | 0 (0) |
| Chronic lymphocytic leukemia | 18%-98% | 31 | 0 (0) |
| CD5-negative mature B-cell neoplasm unclassifiable | 15%-97% | 5 | 0 (0) |
| Healthy blood donors | Not present | 9 | 0 (0) |
AS-PCR, allele-specific PCR; HCL, hairy-cell leukemia; MRD, minimal residual disease.
Including 11 HCL variants.