ASA-induced eicosanoid generation in mouse plasma
| Genotype . | Treatment . | TXB2 . | 6-keto-PGF1α . | 15-epi-LXA4 . |
|---|---|---|---|---|
| (ng/mL) . | ||||
| Fpr2/3+/+ | Sham | 0.91 ± 0.09 | 0.14 ± 0.09 | ND |
| IR | 3.45 ± 0.18* | 3.28 ± 0.65* | ND | |
| IR + ASA | 2.07 ± 0.03*† | 0.87 ± 0.44† | 483 ± 252 | |
| Fpr2/3−/− | Sham | 1.09 ± 0.26 | 0.59 ± 0.17 | ND |
| IR | 2.67 ± 0.88* | 2.01 ± 0.64* | ND | |
| IR + ASA | 2.15 ± 0.03*† | 0.16 ± 0.08† | 789 ± 61 | |
| Genotype . | Treatment . | TXB2 . | 6-keto-PGF1α . | 15-epi-LXA4 . |
|---|---|---|---|---|
| (ng/mL) . | ||||
| Fpr2/3+/+ | Sham | 0.91 ± 0.09 | 0.14 ± 0.09 | ND |
| IR | 3.45 ± 0.18* | 3.28 ± 0.65* | ND | |
| IR + ASA | 2.07 ± 0.03*† | 0.87 ± 0.44† | 483 ± 252 | |
| Fpr2/3−/− | Sham | 1.09 ± 0.26 | 0.59 ± 0.17 | ND |
| IR | 2.67 ± 0.88* | 2.01 ± 0.64* | ND | |
| IR + ASA | 2.15 ± 0.03*† | 0.16 ± 0.08† | 789 ± 61 | |
Blood was collected from sham mice or animals subjected to 30-min clamping of the superior mesenteric artery followed by 90-min IR. Some mice received ASA (100 mg/kg orally 1 h before beginning of ischemia). Plasma was prepared and analyzed for eicosanoids content using specific enzyme immunoassays (see “Methods”). Data are mean ± SEM of 4 samples per group.
ND, not detectable.
P < .05 vs sham.
P < .05 vs respective IR value.