Autoreacive membrane-associated antigens identified by mass spectrometry
| Coomassie gel . | Band ID . | CLL patient . | Protein ID . | Predicted Mw (kD) . | GenBank . |
|---|---|---|---|---|---|
| Gel A | 1 | C | hnRNP U | 90 | 32358 |
| ICAM 3 | 60 | 32628 | |||
| Platelet Glycoprotein lib | 71 | 35520 | |||
| l-Plastin (LCPl) | 71 | 62898171 | |||
| 2 | A | Unnamed Prot. | 71 | 189054552 | |
| hnRNP R | 71 | 5031755 | |||
| Unnamed Prot. | 71 | 35218 | |||
| 3 | D | None Identified | — | — | |
| 4 | E | l-Plastin (LCP1) | 71 | 62898171 | |
| Unnamed Prot. | 66 | 1890541782 | |||
| Unnamed Prot. | 71 | 189054552 | |||
| 5 | C | Trans. Upreg Nuclear Prot. | 51 | 4607892 | |
| Unnamed Prot. | 66 | 189054178 | |||
| T-complex Prot. | 57 | 58761484 | |||
| Gel B | 6 | G | Hypothetical Prot. | 78 | 52545896 |
| hnRNP U | 90 | 32358 | |||
| Splicing Factor 3A | 89 | 5032087 | |||
| Matrin 3 | 95 | 21626466 | |||
| 7 | F | Unnamed Prot. | 71 | 18905552 | |
| Unnamed Prot. | 64 | 194373695 | |||
| 8 | H | Lamin B1 | 67 | 5031755 | |
| Unnamed Prot. | 61 | 32356 | |||
| 9 | F | ICAM 2 | 31 | 32624 | |
| Vimentin | 54 | 340219 | |||
| ATP synthase | 60 | 4757810 | |||
| Coronin-like Prot. | 52 | 1002923 | |||
| Nucleosome Assy Prot. | 46 | 4758756 | |||
| Vimentin | 54 | 47115317 | |||
| 10 | G | hnRNP H | 49 | 5031753 | |
| Unnamed Prot. | 51 | 221041944 | |||
| Polyprimidine tract Prot. | 60 | 4506243 | |||
| ICAM 2 | 31 | 32624 | |||
| 11 | H | Calreticulin Precursor | 48 | 4757900 | |
| PDCD4 | 36 | 223673990 | |||
| Lyn | 59 | 4505055 | |||
| Transmembrane Prot. | 24 | 148233642 | |||
| 12 | H | Unnamed Prot. | 27 | 32532 | |
| Elongation Factor 1 | 25 | 4503477 | |||
| Histone H1.2 | 21 | 4885375 |
| Coomassie gel . | Band ID . | CLL patient . | Protein ID . | Predicted Mw (kD) . | GenBank . |
|---|---|---|---|---|---|
| Gel A | 1 | C | hnRNP U | 90 | 32358 |
| ICAM 3 | 60 | 32628 | |||
| Platelet Glycoprotein lib | 71 | 35520 | |||
| l-Plastin (LCPl) | 71 | 62898171 | |||
| 2 | A | Unnamed Prot. | 71 | 189054552 | |
| hnRNP R | 71 | 5031755 | |||
| Unnamed Prot. | 71 | 35218 | |||
| 3 | D | None Identified | — | — | |
| 4 | E | l-Plastin (LCP1) | 71 | 62898171 | |
| Unnamed Prot. | 66 | 1890541782 | |||
| Unnamed Prot. | 71 | 189054552 | |||
| 5 | C | Trans. Upreg Nuclear Prot. | 51 | 4607892 | |
| Unnamed Prot. | 66 | 189054178 | |||
| T-complex Prot. | 57 | 58761484 | |||
| Gel B | 6 | G | Hypothetical Prot. | 78 | 52545896 |
| hnRNP U | 90 | 32358 | |||
| Splicing Factor 3A | 89 | 5032087 | |||
| Matrin 3 | 95 | 21626466 | |||
| 7 | F | Unnamed Prot. | 71 | 18905552 | |
| Unnamed Prot. | 64 | 194373695 | |||
| 8 | H | Lamin B1 | 67 | 5031755 | |
| Unnamed Prot. | 61 | 32356 | |||
| 9 | F | ICAM 2 | 31 | 32624 | |
| Vimentin | 54 | 340219 | |||
| ATP synthase | 60 | 4757810 | |||
| Coronin-like Prot. | 52 | 1002923 | |||
| Nucleosome Assy Prot. | 46 | 4758756 | |||
| Vimentin | 54 | 47115317 | |||
| 10 | G | hnRNP H | 49 | 5031753 | |
| Unnamed Prot. | 51 | 221041944 | |||
| Polyprimidine tract Prot. | 60 | 4506243 | |||
| ICAM 2 | 31 | 32624 | |||
| 11 | H | Calreticulin Precursor | 48 | 4757900 | |
| PDCD4 | 36 | 223673990 | |||
| Lyn | 59 | 4505055 | |||
| Transmembrane Prot. | 24 | 148233642 | |||
| 12 | H | Unnamed Prot. | 27 | 32532 | |
| Elongation Factor 1 | 25 | 4503477 | |||
| Histone H1.2 | 21 | 4885375 |
Unconfirmed peptide identities were obtained from 11 of the 12 bands; l-plastin (AKA: LCP1) was identified twice at the correct size in two different donors. Gel, patient, and band identifiers can be referenced to Figure 1A-D.