Viral proteins identified by proteomic analysis of PU-H71 pull-downs
| Identified proteins . | Molecular weight . | Classification . | Known function(s) . |
|---|---|---|---|
| ORF20 | 28 kDa | Late | UL24 family: induces cell-cycle arrest and apoptosis |
| ORF18 | 29 kDa | Late | Function not known for KSHV: involved in late gene transcription in other herpesviruses |
| ORF64 | 290 kDa | Early | Tegument protein with deubiqutinase activity: suppresses RIG-I–mediated interferon signaling by reducing the ubiquitination of RIG-I |
| ORF K2 | 23 kDa | Early/latent* | vIL-6: viral homolog of interleukin-6 |
| ORF57 | 51 kDa | Early | Messenger RNA biogenesis |
| ORF22 | 81 kDa | Late | Virion envelope glycoprotein |
| ORF72 | 28 kDa | Latent | vCyclin: viral homolog of cyclin D |
| ORF25 | 153 kDa | Late | Major capsid protein |
| ORK9 | 113 kDa | Early | DNA polymerase |
| ORF27 | 32 kDa | Late | Membrane glycoprotein that contributes to intercellular viral spread |
| ORF63 | 104 kDa | Early | Viral homolog of human NLRP1 (blocks innate immune responses/inflammasome) |
| ORF6 | 125 kDa | Early | Multifunctional DNA-binding proteins with roles central to viral DNA replication |
| ORF56 | 96 kDa | Early | Primase |
| ORF4 | 61 kDa | Late | Complement inhibitor: KCP for complement control protein |
| ORF35 | 17 kDa | Early | Induced by RTA, required for in vitro infection |
| ORF21 | 65 kDa | Early | Thymidine kinase |
| ORF19 | 61 kDa | Early | Tegument protein |
| ORF K4.1 | 13 kDa | Early | vMIP-III/vCCL3-CCR4 agonist |
| ORF17.5 | 31 kDa | Early | Assembly/scaffolding protein |
| ORF62 | 36 kDa | Late | Triplex-1 structural protein |
| ORF2 | 23 kDa | Early | vDHFR: viral homolog of dihydrofolate reductase |
| Identified proteins . | Molecular weight . | Classification . | Known function(s) . |
|---|---|---|---|
| ORF20 | 28 kDa | Late | UL24 family: induces cell-cycle arrest and apoptosis |
| ORF18 | 29 kDa | Late | Function not known for KSHV: involved in late gene transcription in other herpesviruses |
| ORF64 | 290 kDa | Early | Tegument protein with deubiqutinase activity: suppresses RIG-I–mediated interferon signaling by reducing the ubiquitination of RIG-I |
| ORF K2 | 23 kDa | Early/latent* | vIL-6: viral homolog of interleukin-6 |
| ORF57 | 51 kDa | Early | Messenger RNA biogenesis |
| ORF22 | 81 kDa | Late | Virion envelope glycoprotein |
| ORF72 | 28 kDa | Latent | vCyclin: viral homolog of cyclin D |
| ORF25 | 153 kDa | Late | Major capsid protein |
| ORK9 | 113 kDa | Early | DNA polymerase |
| ORF27 | 32 kDa | Late | Membrane glycoprotein that contributes to intercellular viral spread |
| ORF63 | 104 kDa | Early | Viral homolog of human NLRP1 (blocks innate immune responses/inflammasome) |
| ORF6 | 125 kDa | Early | Multifunctional DNA-binding proteins with roles central to viral DNA replication |
| ORF56 | 96 kDa | Early | Primase |
| ORF4 | 61 kDa | Late | Complement inhibitor: KCP for complement control protein |
| ORF35 | 17 kDa | Early | Induced by RTA, required for in vitro infection |
| ORF21 | 65 kDa | Early | Thymidine kinase |
| ORF19 | 61 kDa | Early | Tegument protein |
| ORF K4.1 | 13 kDa | Early | vMIP-III/vCCL3-CCR4 agonist |
| ORF17.5 | 31 kDa | Early | Assembly/scaffolding protein |
| ORF62 | 36 kDa | Late | Triplex-1 structural protein |
| ORF2 | 23 kDa | Early | vDHFR: viral homolog of dihydrofolate reductase |
vIL-6 is increased upon lytic reactivation, but is expressed in latently infected PEL.40