Table 1 Quantitation of terminal... | American Society of Hematology

Table 1

Quantitation of terminal erythroid differentiation in normal and anemic mouse models

	Pro (%)	Baso (%)	Poly (%)	Ortho (%)
WT (n = 20)
Bone marrow	6.1 ± 1.2	12.7 ± 1.6	27.7 ± 1.4	53.1 ± 3.0
Spleen	Very low level of erythropoiesis
4.1R KO (n = 6)
Bone marrow	6.5 ± 0.5	13.9 ± 1.2	27.8 ± 1.4	52.2 ± 2.2
Spleen	5.5 ± 1.0	12.7 ± 1.7	27.7 ± 1.8	54.2 ± 2.7
Phlebotomy-induced anemia (n = 6)
Bone marrow	6.9 ± 1.1	14.3 ± 1.3	28.4 ± 2.0	52.0 ± 3.5
Spleen	7.0 ± 1.1	13.7 ± 2.0	26.3 ± 4.9	50.5 ± 5.4
Hbb^th1/th1 (PBS-treated, n = 6)
Bone marrow	4.4 ± 0.3*	15.5 ± 1.5†	26.7 ± 0.9	53.5 ± 1.3
Spleen	4.7 ± 1.1‡	15.1 ± 4.7§	26.6 ± 3.0	53.6 ± 8.4
Hbb^th1/th1 (transferrin-treated n = 6)
Bone marrow	6.6 ± 0.8	13.9 ± 2.7	25.6 ± 2.2	51.3 ± 1.5
Spleen	Very low level of erythropoiesis

	Pro (%)	Baso (%)	Poly (%)	Ortho (%)
WT (n = 20)
Bone marrow	6.1 ± 1.2	12.7 ± 1.6	27.7 ± 1.4	53.1 ± 3.0
Spleen	Very low level of erythropoiesis
4.1R KO (n = 6)
Bone marrow	6.5 ± 0.5	13.9 ± 1.2	27.8 ± 1.4	52.2 ± 2.2
Spleen	5.5 ± 1.0	12.7 ± 1.7	27.7 ± 1.8	54.2 ± 2.7
Phlebotomy-induced anemia (n = 6)
Bone marrow	6.9 ± 1.1	14.3 ± 1.3	28.4 ± 2.0	52.0 ± 3.5
Spleen	7.0 ± 1.1	13.7 ± 2.0	26.3 ± 4.9	50.5 ± 5.4
Hbb^th1/th1 (PBS-treated, n = 6)
Bone marrow	4.4 ± 0.3*	15.5 ± 1.5†	26.7 ± 0.9	53.5 ± 1.3
Spleen	4.7 ± 1.1‡	15.1 ± 4.7§	26.6 ± 3.0	53.6 ± 8.4
Hbb^th1/th1 (transferrin-treated n = 6)
Bone marrow	6.6 ± 0.8	13.9 ± 2.7	25.6 ± 2.2	51.3 ± 1.5
Spleen	Very low level of erythropoiesis

The proportion of cells at each distinct stage of maturation is normalized based on total nucleated erythroblasts as 100%.

WT indicates wild-type.

P = .0026 WT versus Hbb^th1/th1 mice.

P = .0023 WT versus Hbb^th1/th1 mice.

P = .033 WT versus Hbb^th1/th1 mice.

P = .042 WT versus Hbb^th1/th1 mice.