Table 1

Tetramer binding using univalent and divalent recombinant proteins

RecombinantUnivalent Kd (ITC)*Divalent Kd (SE)
23°C30°C23°C30°C
WT 0.5 μM 1.8 μM 0.1 μM 1 μM 
L260P 0.4 μM 0.8 μM 1.2 μM 5 μM 
Q471P 0.4 μM 0.7 μM 0.2 μM 1 μM 
RecombinantUnivalent Kd (ITC)*Divalent Kd (SE)
23°C30°C23°C30°C
WT 0.5 μM 1.8 μM 0.1 μM 1 μM 
L260P 0.4 μM 0.8 μM 1.2 μM 5 μM 
Q471P 0.4 μM 0.7 μM 0.2 μM 1 μM 

ITC, isothermal titration calorimetry; SE, sedimentation equilibrium.

*

ITC was used to measure the univalent binding of WT, L260P, or Q471P α0-5 titrated into β16-17.

SE was used to measure the divalent dimer-tetramer equilibrium of WT, L260P, and Q471P mini-spectrins.

or Create an Account

Close Modal
Close Modal