List of macrophage models used in the study
| Dataset . | Maturation stimulus/dose duration . | Activation stimulus/dose duration . |
|---|---|---|
| Human dataset 1 (hMΦ1) | M-CSF 100 ng/mL/7 d | IL-4 20 ng/mL/18 h |
| Human dataset 2 (hMΦ2) | 1% Autologous serum/7 d | IL-4 20 ng/mL/24 h |
| Human dataset 3 (hMΦ3) | 1% Autologous serum/7 d | IL-4 10 ng/mL/48 h |
| Human dataset 4 (hMΦ4) | 10% Autologous serum/7 d | IL-4 20 ng/mL/7 d |
| Human dataset 5 (hMΦ5) | 10% Autologous serum/7 d | 50 ng/mL of IL-4 or 50 U/mL of IFN-γ + 12.5 ng/mL of TNF-α or 50 ng/mL of M-CSF or 50 ng/mL of IL-10/5 d |
| Mouse dataset mBMMΦ | L-cell–conditioned medium (M-CSF)/7 d | IL-4 20 ng/mL/18 h |
| Mouse datasets ThioMΦ1, ThioMΦ2, and BioMΦ* | Thioglycollate or Biogel/4 d | mIL-4/X63Ag8-653 supernatant/48 h |
| Mouse dataset (ThioMΦ3) | Thioglycollate/4 d | IL-4 10 ng/mL/48 h |
| Dataset . | Maturation stimulus/dose duration . | Activation stimulus/dose duration . |
|---|---|---|
| Human dataset 1 (hMΦ1) | M-CSF 100 ng/mL/7 d | IL-4 20 ng/mL/18 h |
| Human dataset 2 (hMΦ2) | 1% Autologous serum/7 d | IL-4 20 ng/mL/24 h |
| Human dataset 3 (hMΦ3) | 1% Autologous serum/7 d | IL-4 10 ng/mL/48 h |
| Human dataset 4 (hMΦ4) | 10% Autologous serum/7 d | IL-4 20 ng/mL/7 d |
| Human dataset 5 (hMΦ5) | 10% Autologous serum/7 d | 50 ng/mL of IL-4 or 50 U/mL of IFN-γ + 12.5 ng/mL of TNF-α or 50 ng/mL of M-CSF or 50 ng/mL of IL-10/5 d |
| Mouse dataset mBMMΦ | L-cell–conditioned medium (M-CSF)/7 d | IL-4 20 ng/mL/18 h |
| Mouse datasets ThioMΦ1, ThioMΦ2, and BioMΦ* | Thioglycollate or Biogel/4 d | mIL-4/X63Ag8-653 supernatant/48 h |
| Mouse dataset (ThioMΦ3) | Thioglycollate/4 d | IL-4 10 ng/mL/48 h |
All assays were done in biological triplicates except that for mouse datasets ThioMΦ1, ThioMΦ2, and BioMΦ, which was performed in single using pooled RNA.