CPL scan parameters
| CPL ID . | Sequence of position one sublibraries . | Total no. of peptides in library (a + 19) × 19n . | No. of sublibraries . | No. of peptides in each sublibrary (19n) . | Concentration of each peptide in sublibrary* . |
|---|---|---|---|---|---|
| 8mer | OXXXXXXX | 2.4 × 1010 | 160 | 8.9 × 108 | 1.1 × 10−13 M |
| 9mer | OXXXXXXXX | 4.8 × 1011 | 180 | 1.7 × 1010 | 5.9 × 10−15 M |
| 10mer | OXXXXXXXXX | 9.4 × 1012 | 200 | 3.2 × 1011 | 3.1 × 10−16 M |
| 11mer | OXXXXXXXXXX | 1.8 × 1014 | 220 | 6.1 × 1012 | 1.6 × 10−17 M |
| 12mer | OXXXXXXXXXXX | 3.6 × 1015 | 240 | 1.2 × 1014 | 8.6 × 10−19 M |
| 13mer | OXXXXXXXXXXXX | 7.1 × 1016 | 260 | 2.2 × 1015 | 4.5 × 10−20 M |
| CPL ID . | Sequence of position one sublibraries . | Total no. of peptides in library (a + 19) × 19n . | No. of sublibraries . | No. of peptides in each sublibrary (19n) . | Concentration of each peptide in sublibrary* . |
|---|---|---|---|---|---|
| 8mer | OXXXXXXX | 2.4 × 1010 | 160 | 8.9 × 108 | 1.1 × 10−13 M |
| 9mer | OXXXXXXXX | 4.8 × 1011 | 180 | 1.7 × 1010 | 5.9 × 10−15 M |
| 10mer | OXXXXXXXXX | 9.4 × 1012 | 200 | 3.2 × 1011 | 3.1 × 10−16 M |
| 11mer | OXXXXXXXXXX | 1.8 × 1014 | 220 | 6.1 × 1012 | 1.6 × 10−17 M |
| 12mer | OXXXXXXXXXXX | 3.6 × 1015 | 240 | 1.2 × 1014 | 8.6 × 10−19 M |
| 13mer | OXXXXXXXXXXXX | 7.1 × 1016 | 260 | 2.2 × 1015 | 4.5 × 10−20 M |
n indicates the number of degenerate positions; O, fixed sequence position (1 of the 20 proteogenic L-amino acids; O is moved systematically through the peptide backbone in a full CPL); X, degenerate position (1 of 19 proteogenic L-amino acids, excluding cysteine); and a, full peptide length.
When mixtures are used at a concentration of 100μM.