Increased expression of costimulatory molecules on miR-142 deficient DCs
| Surface marker . | Splenic DC subset . | miR-142+/+ . | miR-142−/− . | t test . |
|---|---|---|---|---|
| CD40 | CD4+ DC | 942.3 ± 86.1 | 1082.2 ± 53.7 | .012 |
| CD8a+ DC | 1513.5 ± 160.1 | 1790.2 ± 66.0 | .006 | |
| DN DC | 827.8 ± 53.8 | 801.0 ± 96.2 | .572 | |
| CD80 | CD4+ DC | 1160.7 ± 81.5 | 1865.2 ± 162.9 | .00001 |
| CD8a+ DC | 961.5 ± 119.1 | 784.0 ± 84.8 | .021 | |
| DN DC | 758.7 ± 83.9 | 982.4 ± 77.0 | .001 | |
| CD86 | CD4+ DC | 233.8 ± 65.3 | 345.8 ± 30.9 | .007 |
| CD8a+ DC | 365.7 ± 27.4 | 517.0 ± 15.7 | 2 × 10−6 | |
| DN DC | 155.7 ± 67.0 | 197.0 ± 18.1 | .217 | |
| MHCII | CD4+ DC | 10 811.7 ± 1865.0 | 7037.8 ± 708.7 | .002 |
| CD8a+ DC | 7372 ± 1667.5 | 6206.4 ± 620.8 | .176 | |
| DN DC | 8878.5 ± 1380.1 | 5150.6 ± 536.9 | .0003 |
| Surface marker . | Splenic DC subset . | miR-142+/+ . | miR-142−/− . | t test . |
|---|---|---|---|---|
| CD40 | CD4+ DC | 942.3 ± 86.1 | 1082.2 ± 53.7 | .012 |
| CD8a+ DC | 1513.5 ± 160.1 | 1790.2 ± 66.0 | .006 | |
| DN DC | 827.8 ± 53.8 | 801.0 ± 96.2 | .572 | |
| CD80 | CD4+ DC | 1160.7 ± 81.5 | 1865.2 ± 162.9 | .00001 |
| CD8a+ DC | 961.5 ± 119.1 | 784.0 ± 84.8 | .021 | |
| DN DC | 758.7 ± 83.9 | 982.4 ± 77.0 | .001 | |
| CD86 | CD4+ DC | 233.8 ± 65.3 | 345.8 ± 30.9 | .007 |
| CD8a+ DC | 365.7 ± 27.4 | 517.0 ± 15.7 | 2 × 10−6 | |
| DN DC | 155.7 ± 67.0 | 197.0 ± 18.1 | .217 | |
| MHCII | CD4+ DC | 10 811.7 ± 1865.0 | 7037.8 ± 708.7 | .002 |
| CD8a+ DC | 7372 ± 1667.5 | 6206.4 ± 620.8 | .176 | |
| DN DC | 8878.5 ± 1380.1 | 5150.6 ± 536.9 | .0003 |
Mean fluorescence intensity (MFI) analysis of the co-stimulatory molecules CD40, CD80, and CD86 on splenic classical DCs isolated from miR-142−/− mice and littermate controls. At least 5 animals per group were used for the analysis. P < .05 considered to be significant using the Student 2-tailed t test.