Sequences for HIV DNA and GAPDH primers and probes
| Target . | Sequence . |
|---|---|
| HIV-US1 strong-stop | F: 5′-AACTAGGGAACCCACTGCTTAA |
| R: 5′-TGAGGGATCTCTAGTTACCAGAGTCA | |
| Probe: 5′- (FAM) CCTCAATAAAGCTTGCCTTGAGTGCTTCAA | |
| HIV-US1 full-length | F: 5′-AACTAGGGAACCCACTGCTTAA |
| R: 5′-CGAGTCCTGCGTCGAGAGA | |
| Probe: 5′-CCTCAATAAAGCTTGCCTTGAGTGCTTCAA | |
| GAPDH | F: 5′-ACCGGGAAGGAAATGAATGG |
| R: 5′-GCAGGAGCGCAGGGTTAGT | |
| Probe: 5′ (VIC) ACCGGCAGGCTTTCCTAACGGCT |
| Target . | Sequence . |
|---|---|
| HIV-US1 strong-stop | F: 5′-AACTAGGGAACCCACTGCTTAA |
| R: 5′-TGAGGGATCTCTAGTTACCAGAGTCA | |
| Probe: 5′- (FAM) CCTCAATAAAGCTTGCCTTGAGTGCTTCAA | |
| HIV-US1 full-length | F: 5′-AACTAGGGAACCCACTGCTTAA |
| R: 5′-CGAGTCCTGCGTCGAGAGA | |
| Probe: 5′-CCTCAATAAAGCTTGCCTTGAGTGCTTCAA | |
| GAPDH | F: 5′-ACCGGGAAGGAAATGAATGG |
| R: 5′-GCAGGAGCGCAGGGTTAGT | |
| Probe: 5′ (VIC) ACCGGCAGGCTTTCCTAACGGCT |