Summary of antigens targeted by CLL-derived Fab, binding epitope, IG heavy/light chain usage, mutational status, and stereotyped subsets
| No. . | Antigen . | Accession no. . | BCR-binding epitope . | Recognized by patient no. . | Heavy chain . | Light chain . | Germline homology (IGHV/IGKV/IGLV) (%) . | Mutations . | Subset . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IGHV . | IGDV . | Frame . | IGJV . | Type . | IGKV/IGLV . | IGKJ/IGLJ . | ||||||||
| 1 | Pyruvate carboxylase | NM_000920 | aa1031-1084 | 24 | 4-34*01 | 1-14*01 | 1 | 4*02 | IGKV | 3-20*01 | 1*01 | 98.6/98.58 | Unmut./unmut. | None |
| aa1124-1181 | 26 | 3-7*03 | 3-3*01 | 1 | 4*02 | IGLV | 4-69*01 | 2*01 | 100/100 | Unmut./unmut. | None | |||
| 2 | LLP homolog | NM_032338 | aa38-91 | 20 | 5-51*01 | 3-3*01 | 2 | 6*02 | IGKV | 1-5*01 | 4*01 | 100/96.77 | Unmut./mut. | None |
| 3 | Fam32A | NM_014077 | aa1-32 | 21 | 1-69*01 | 3-10*01 | 3 | 6*03 | IGLV | 3-21*02 | 2*01 | 100/100 | Unmut./unmut. | 5 |
| aa69-79 | 31 | 3-23*04 | 3-3*01 | 2 | 1*01 | IGKV | 3-20*01 | 1*01 | 98.26/98.23 | Unmut./unmut. | None | |||
| 4 | Notch2 | NM_024408 | aa495-505 | 51 | 3-23*01 | 4-23*01 | 3 | 4*02 | IGKV | 3-15*01 | 4*01 | 97.1/100 | Mut./unmut. | None |
| 5 | CACYBP | NM_014412 | aa92-102 | 44 | 4-34*01 | 3-10*01 | 2 | 6*02 | IGKV | 2-30*01 | 2*01 | 96.14/97.96 | Mut./mut. | 4 |
| aa1-102 | 46 | 3-30*03F | 3-3*01F | 2 | 4*02 | IGKV | 3-11*01 | 2*01 | 91.67/96.42 | Mut./mut. | None | |||
| 6 | SMCHD1 | NM_015295 | aa1897-1907 | 45 | 3-30*03 | 3-09*01 | 1 | 4*02 | IGLV | 2-14*02 | 3*01 | 100/100 | Unmut./unmut. | None |
| 7 | MAZ | NM_001042539 | aa239-254 | 27 | 4-34*03F | 3-3*01F | 2 | 6*02 | IGKV | 3-20*01F | 1*01F | 95.77/96.1 | Mut./mut. | None |
| 8 | Actin-γ | NM_001614.21 | aa55-67 | 47 | 3-13*01 | 6-19*01 | 1 | 2*01 | IGKV | 3-11*01 | 3*01 | 100/100 | Unmut./unmut. | None |
| 9 | MTUS1 | NM_001001924 | aa1146-1162 | 48 | 1-24*01 | 6-19*01 | 1 | 6*02 | IGKV | 3-11*01 | 1*01 | 100/100 | Unmut./unmut. | None |
| No. . | Antigen . | Accession no. . | BCR-binding epitope . | Recognized by patient no. . | Heavy chain . | Light chain . | Germline homology (IGHV/IGKV/IGLV) (%) . | Mutations . | Subset . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IGHV . | IGDV . | Frame . | IGJV . | Type . | IGKV/IGLV . | IGKJ/IGLJ . | ||||||||
| 1 | Pyruvate carboxylase | NM_000920 | aa1031-1084 | 24 | 4-34*01 | 1-14*01 | 1 | 4*02 | IGKV | 3-20*01 | 1*01 | 98.6/98.58 | Unmut./unmut. | None |
| aa1124-1181 | 26 | 3-7*03 | 3-3*01 | 1 | 4*02 | IGLV | 4-69*01 | 2*01 | 100/100 | Unmut./unmut. | None | |||
| 2 | LLP homolog | NM_032338 | aa38-91 | 20 | 5-51*01 | 3-3*01 | 2 | 6*02 | IGKV | 1-5*01 | 4*01 | 100/96.77 | Unmut./mut. | None |
| 3 | Fam32A | NM_014077 | aa1-32 | 21 | 1-69*01 | 3-10*01 | 3 | 6*03 | IGLV | 3-21*02 | 2*01 | 100/100 | Unmut./unmut. | 5 |
| aa69-79 | 31 | 3-23*04 | 3-3*01 | 2 | 1*01 | IGKV | 3-20*01 | 1*01 | 98.26/98.23 | Unmut./unmut. | None | |||
| 4 | Notch2 | NM_024408 | aa495-505 | 51 | 3-23*01 | 4-23*01 | 3 | 4*02 | IGKV | 3-15*01 | 4*01 | 97.1/100 | Mut./unmut. | None |
| 5 | CACYBP | NM_014412 | aa92-102 | 44 | 4-34*01 | 3-10*01 | 2 | 6*02 | IGKV | 2-30*01 | 2*01 | 96.14/97.96 | Mut./mut. | 4 |
| aa1-102 | 46 | 3-30*03F | 3-3*01F | 2 | 4*02 | IGKV | 3-11*01 | 2*01 | 91.67/96.42 | Mut./mut. | None | |||
| 6 | SMCHD1 | NM_015295 | aa1897-1907 | 45 | 3-30*03 | 3-09*01 | 1 | 4*02 | IGLV | 2-14*02 | 3*01 | 100/100 | Unmut./unmut. | None |
| 7 | MAZ | NM_001042539 | aa239-254 | 27 | 4-34*03F | 3-3*01F | 2 | 6*02 | IGKV | 3-20*01F | 1*01F | 95.77/96.1 | Mut./mut. | None |
| 8 | Actin-γ | NM_001614.21 | aa55-67 | 47 | 3-13*01 | 6-19*01 | 1 | 2*01 | IGKV | 3-11*01 | 3*01 | 100/100 | Unmut./unmut. | None |
| 9 | MTUS1 | NM_001001924 | aa1146-1162 | 48 | 1-24*01 | 6-19*01 | 1 | 6*02 | IGKV | 3-11*01 | 1*01 | 100/100 | Unmut./unmut. | None |
Mut., mutated; unmut., unmutated.
*Samples with less than 2% nucleotide differences to published germline sequences were defined as unmutated.