Occurrence of nSNVs in the TP53 3′UTR potentially affecting miRNA binding in DLBCL
| Subgroup . | No. of patients (%) . | ||
|---|---|---|---|
| Training Set 76 of 244 . | Validation Set 70 of 247 . | Total 146 of 491 . | |
| TP53 CDS status | |||
| MUT-CDS | 16 (21) | NA | NA |
| WT-CDS | 60 (79) | NA | NA |
| 3′UTR nSNV affecting putative miRNA binding* | |||
| Generating only | 12 | 5 | 17 |
| Disrupting only | 31 | 23 | 54 |
| Generating and disrupting | 15 | 9 | 24 |
| Others | 18 | 33 | 51 |
| No. of 3′UTR nucleotides altered | |||
| 1 | 42 (59.5) | 42 (60) | 84 (57.5) |
| 2 | 19 (23.8) | 18 (25.7) | 37 (25.3) |
| 3 | 8 (9.5) | 7 (10) | 15 (10.3) |
| 4 | 5 (4.8) | 3 (4.3) | 8 (5.5) |
| 5 | 1 (1.2) | 0 | 1 (0.7) |
| 6 | 1 (1.2) | 0 | 1 (0.7) |
| Subgroup . | No. of patients (%) . | ||
|---|---|---|---|
| Training Set 76 of 244 . | Validation Set 70 of 247 . | Total 146 of 491 . | |
| TP53 CDS status | |||
| MUT-CDS | 16 (21) | NA | NA |
| WT-CDS | 60 (79) | NA | NA |
| 3′UTR nSNV affecting putative miRNA binding* | |||
| Generating only | 12 | 5 | 17 |
| Disrupting only | 31 | 23 | 54 |
| Generating and disrupting | 15 | 9 | 24 |
| Others | 18 | 33 | 51 |
| No. of 3′UTR nucleotides altered | |||
| 1 | 42 (59.5) | 42 (60) | 84 (57.5) |
| 2 | 19 (23.8) | 18 (25.7) | 37 (25.3) |
| 3 | 8 (9.5) | 7 (10) | 15 (10.3) |
| 4 | 5 (4.8) | 3 (4.3) | 8 (5.5) |
| 5 | 1 (1.2) | 0 | 1 (0.7) |
| 6 | 1 (1.2) | 0 | 1 (0.7) |
Others, outside seed match or not in miRNA sites; NA, not applicable.
nSNVs predicted by TargetScan 5.0 to be targeted by miRNAs (based on the seed sequence of the miRNA plus A1 and m8). Some patients had both mutations generating miRNA binding and mutations disrupting it. Three SNPs listed in dbSNP that putatively affect miRNA binding (rs16956880 [205 G>A], rs11483 1472 [1070 G>A], and rs78378222 [1175 C>A]) were excluded.