List of LAM-PCR integration analysis sites for STEMCCA-loxP
| iPSC line . | Chromosome . | bp position . | Gene . |
|---|---|---|---|
| iNC-01 | Chr 17 | 76054451 | TNRC6C (intron) |
| iGP91-02 | Chr 6 | 147435589 | ENSG00000233452 |
| iGP91-03-1 | Chr 1 | 44312073 | ST3GAL3 (intron) |
| iGP91-03-2 | Chr 22 | 21115470 | PI4KA (intron) |
| iGP91-04 | Chr 17 | 3618824 | ITGAE (intron) |
| iP47-01 | Chr 3 | 112952023 | BOC (intron) |
| iP47-02 | Chr 1 | 39677115 | MACF1 (intron) |
| iPSC line . | Chromosome . | bp position . | Gene . |
|---|---|---|---|
| iNC-01 | Chr 17 | 76054451 | TNRC6C (intron) |
| iGP91-02 | Chr 6 | 147435589 | ENSG00000233452 |
| iGP91-03-1 | Chr 1 | 44312073 | ST3GAL3 (intron) |
| iGP91-03-2 | Chr 22 | 21115470 | PI4KA (intron) |
| iGP91-04 | Chr 17 | 3618824 | ITGAE (intron) |
| iP47-01 | Chr 3 | 112952023 | BOC (intron) |
| iP47-02 | Chr 1 | 39677115 | MACF1 (intron) |
Shown are the single-copy STEMCCA-loxP integration sites in 7 independent iPSC lines determined by LAM-PCR before Cre-excision and verified by flanking genome primer PCR facilitated sequencing after Cre-excision. The chromosome, base pair position within the chromosome, and gene location in which insertion occurred (when indicated) were determined using ENSEMBL release 67, Homo sapiens high coverage assembly GRCh37 (GCA_000001405.6) from the Genome Reference Consortium. The 303-bp residual STEMCCA-loxP footprint sequence at the indicated genomic site was identical for all 7 clones analyzed by LAM-PCR and postexcision sequencing, as shown in Figure 2. The line source name is as designated in Table 1. Note that in the case of donor source iGP91-03, 2 clones (iGP91-03-1 and iGP91-03-2) arising in the same culture from that reprogramming experiment were analyzed.
Chr, chromosome.