Details of primary antibodies used for immunohistochemistry
| Primary antibody . | Supplier/code . | Species (clone) . | Antigen retrieval . | Concentration . | Incubation . |
|---|---|---|---|---|---|
| P110α | Cell Signaling#4249 | Rabbit polyclonal(C73F8) | Citrate pH6 | 1:250 | 40 seconds |
| P110β | Abcamab55593 | Mouse monoclonal | Citrate pH6 | 1:250 | 40 seconds |
| P110δ | Santa Cruzsc-55589 | Mouse monoclonal (A-8) | Citrate pH6 | 1:500 | 40 seconds |
| PTEN | CascadeABM-2052 | Mouse monoclonal (6H2.1) | Citrate pH6 | 1:25 | 60 seconds |
| Primary antibody . | Supplier/code . | Species (clone) . | Antigen retrieval . | Concentration . | Incubation . |
|---|---|---|---|---|---|
| P110α | Cell Signaling#4249 | Rabbit polyclonal(C73F8) | Citrate pH6 | 1:250 | 40 seconds |
| P110β | Abcamab55593 | Mouse monoclonal | Citrate pH6 | 1:250 | 40 seconds |
| P110δ | Santa Cruzsc-55589 | Mouse monoclonal (A-8) | Citrate pH6 | 1:500 | 40 seconds |
| PTEN | CascadeABM-2052 | Mouse monoclonal (6H2.1) | Citrate pH6 | 1:25 | 60 seconds |