Table 2 SPDP-modified rFXIII-A 2 *... | American Society of Hematology

Table 2

SPDP-modified rFXIII-A₂* peptides identified by LC-MS/MS following tryptic digest of the rFXIII-A₂*/peptide complex

Fibrin αC peptide used	rFXIII-A₂ SPDP-modified fragment identified	rFXIII-A₂ fragment region	rFXIII-A₂ SPDP-modified lysine	Mass error (Da)	Ion score	Expect
Pep1-Cys*	KDGTHVVENVDATHIGK	446-462	446	0.0105	20	0.011
Cys-Pep1†	KDGTHVVENVDATHIGK	446-462	446	0.00158	15	0.031
	GNPIKVSR	253-260	257	–0.0005	41	8.40E-05
	YPQENKGTYIPVPIVSELQSGK	108-129	113	–0.00089	18	0.015

Fibrin αC peptide used	rFXIII-A₂ SPDP-modified fragment identified	rFXIII-A₂ fragment region	rFXIII-A₂ SPDP-modified lysine	Mass error (Da)	Ion score	Expect
Pep1-Cys*	KDGTHVVENVDATHIGK	446-462	446	0.0105	20	0.011
Cys-Pep1†	KDGTHVVENVDATHIGK	446-462	446	0.00158	15	0.031
	GNPIKVSR	253-260	257	–0.0005	41	8.40E-05
	YPQENKGTYIPVPIVSELQSGK	108-129	113	–0.00089	18	0.015

SPDP-modified lysine residues are shown in bold and underlined in the amino acid fragment sequence. Sequence coverage by LC-MS/MS was 48% for Pep1-Cys and 39% for Cys-Pep1.

Pep1-Cys PDWGTFEEVSGNVSC.

†

Cys-Pep1 CPDWGTFEEVSGNVS.

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