Clonal frequency analysis of DN2 cells
| . | B . | NK . | Myeloid . |
|---|---|---|---|
| GATA3+/− DN2 (OP9) | |||
| IL-2 | ND | 2.85 (2.31-3.53) | ND |
| IL-7 + Flt3L | ND | 5.33 (4.24-6.70) | ND |
| GM-CSF + IL-4 | ND | ND | ND |
| GATA3−/− DN2 (OP9) | |||
| IL-2 | 11.24 (8.47-14.91) | 12.79 (9.51-17.21) | ND |
| IL-7 + Flt3L | 6.57 (5.17-8.35) | 77.46 (40.18-149.32) | ND |
| GM-CSF + IL-4 | 9.56 (6.25-14.6) | 11.49 (5.48-24.1) | 4915 (691-34 948) |
| . | B . | NK . | Myeloid . |
|---|---|---|---|
| GATA3+/− DN2 (OP9) | |||
| IL-2 | ND | 2.85 (2.31-3.53) | ND |
| IL-7 + Flt3L | ND | 5.33 (4.24-6.70) | ND |
| GM-CSF + IL-4 | ND | ND | ND |
| GATA3−/− DN2 (OP9) | |||
| IL-2 | 11.24 (8.47-14.91) | 12.79 (9.51-17.21) | ND |
| IL-7 + Flt3L | 6.57 (5.17-8.35) | 77.46 (40.18-149.32) | ND |
| GM-CSF + IL-4 | 9.56 (6.25-14.6) | 11.49 (5.48-24.1) | 4915 (691-34 948) |
Gata3+/− and Gata3−/− DN2 precursors were cocultured with OP9 stroma under the indicated conditions and analyzed after 2 weeks.
Values represent frequencies (1/x) with 95% confidence intervals. Frequencies were calculated according to Hu and Smyth.30
ND, not detected.