List of primary antibodies and antigen retrieval methodologies
| Antibody . | Clone/manufacturer and catalog no. . | Antigen-retrieval pretreatment . | Antibody dilution . | Species . |
|---|---|---|---|---|
| Desmin | D33/Lab Vision; no. MS-376-S1 | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/200 | Mouse |
| Desmin | Polyclonal/Lab Vision; no. RB-9014-P1 | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/200 | Rabbit |
| CD35 | Ber-MAC-DRC/Dako North America; no. M0846 | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/100 | Mouse |
| CD21 | 1F8/Dako North America; no. M0784 | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/100 | Mouse |
| IL-7 | 7417/R&D Systems; no. MAB207 | Diva Decloaker; high pressure cooker for 30 seconds at 125°C Proteinase K treatment for 15 minutes | 1/100 | Mouse |
| Lymphotoxin-β | 135105/R&D Systems; no. MAB1684 | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/100 | Mouse |
| CD4 | Polyclonal/R&D Systems; no. AF-379-NA | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/100 | Goat |
| CD4 | 1F6/Novacastra; no. NCL-CD4-1F6 | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/100 | Mouse |
| CD8 | SP16/Neomarkers; no. RM-9116-s | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/100 | Rabbit |
| Lymphotoxin-β | Polyclonal/Santa Cruz Biotechnology; no. sc-23561 | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/100 | Goat |
| In Situ Cell Death Detection Kit | Roche Applied Science; no. 1 684 817 | |||
| IgG isotype controls | Dako North America, Jackson ImmunoResearch Laboratories | Diva Decloaker; high pressure cooker for 30 seconds at 125°C Protease K (10 μg/mL) | 1/100 | Mouse, rabbit, goat |
| Antibody . | Clone/manufacturer and catalog no. . | Antigen-retrieval pretreatment . | Antibody dilution . | Species . |
|---|---|---|---|---|
| Desmin | D33/Lab Vision; no. MS-376-S1 | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/200 | Mouse |
| Desmin | Polyclonal/Lab Vision; no. RB-9014-P1 | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/200 | Rabbit |
| CD35 | Ber-MAC-DRC/Dako North America; no. M0846 | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/100 | Mouse |
| CD21 | 1F8/Dako North America; no. M0784 | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/100 | Mouse |
| IL-7 | 7417/R&D Systems; no. MAB207 | Diva Decloaker; high pressure cooker for 30 seconds at 125°C Proteinase K treatment for 15 minutes | 1/100 | Mouse |
| Lymphotoxin-β | 135105/R&D Systems; no. MAB1684 | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/100 | Mouse |
| CD4 | Polyclonal/R&D Systems; no. AF-379-NA | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/100 | Goat |
| CD4 | 1F6/Novacastra; no. NCL-CD4-1F6 | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/100 | Mouse |
| CD8 | SP16/Neomarkers; no. RM-9116-s | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/100 | Rabbit |
| Lymphotoxin-β | Polyclonal/Santa Cruz Biotechnology; no. sc-23561 | Diva Decloaker; high pressure cooker for 30 seconds at 125°C | 1/100 | Goat |
| In Situ Cell Death Detection Kit | Roche Applied Science; no. 1 684 817 | |||
| IgG isotype controls | Dako North America, Jackson ImmunoResearch Laboratories | Diva Decloaker; high pressure cooker for 30 seconds at 125°C Protease K (10 μg/mL) | 1/100 | Mouse, rabbit, goat |