FACS sorting and T-cell cloning results
| UCB no. . | FACS sort definitions . | No. of growing clones . | No. of HY tetramer-positive T-cell clones . | No. of clones with lysis* of male natural ligand . | No. of clones with lysis* of peptide-loaded cells . | HY microchimerism in cell subset . |
|---|---|---|---|---|---|---|
| 1 | CD8+HYA2 | 80 | 8 | 1 | 5 | nt |
| 2 | CD8+HYA2 | 24 | 3 | 1 | 1 | nt |
| 3 | CD8+HYA2 | 24 | 5 | 0 | 2 | nt |
| 4 | CD8+HYA2 | 27 | 10 | 0 | 4 | nt |
| 5 | CD8+HYA2 | 47 | 11 | 3 | 6 | Negative |
| CD8+HYB7 | 12 | 1 | 0 | 0 | ||
| 6 | CD8+HYA2 | 25 | 8 | 3 | 7 | nt |
| 7 | CD8+HYA2CD45RO+ | 27 | 1 | 0 | 1 | Monocytes |
| CD8+HYA2CD45RO− | 48 | 3 | 1 | 1 | ||
| 8 | CD8+HYA2 | 8 | 0 | na | na | nt |
| 9 | CD8+HYA2CD45RO+ | 64 | 1 | 0 | 1 | mDC, B cell |
| 10 | CD8+HYA2 | 99 | 2 | 1 | 0 | B cell |
| CD8+HYB7 | 95 | 9 | 0 | 1 | ||
| 11 | CD8+HYA2 | 31 | 1 | 0 | 1 | Negative |
| CD8+HYA2CD45RO+ | 3 | 0 | na | na | ||
| 12 | CD8+HYA2HYB7HYB60 | 80 | HYA2: 2 | 0 | 2 | nt |
| HYB7: 1 | 1 | 1 | ||||
| HYB60: 0 | na | na | ||||
| 13 | nt | na | na | na | na | Negative |
| 14 | nt | na | na | na | na | mDC |
| 15 | nt | na | na | na | na | mDC |
| 16 | nt | na | na | na | na | Monocytes |
| Total, n = | 12 | 694 | 66 of 694 tested | 11 | 33 | 6 of 9 tested |
| 17 | CD8+HYA2 | 5 | 2 | 1 | 2 | nt |
| 18 | CD8+HYA2 | 25 | 5 | 1 | 1 | nt |
| 19 | CD8+HYA2 | 81 | 1 | 0 | 0 | nt |
| 20 | CD8+HYA2 | 234 | 13 | 2 | 3 | Negative |
| 21 | CD8+HYB7 | 70 | 17 | 2 | 3 | nt |
| 22 | nt | na | na | na | na | Negative |
| 23 | nt | na | na | na | na | Negative |
| Total, n = | 5 | 415 | 38 of 415 tested | 6 | 9 | 0 of 3 tested |
| UCB no. . | FACS sort definitions . | No. of growing clones . | No. of HY tetramer-positive T-cell clones . | No. of clones with lysis* of male natural ligand . | No. of clones with lysis* of peptide-loaded cells . | HY microchimerism in cell subset . |
|---|---|---|---|---|---|---|
| 1 | CD8+HYA2 | 80 | 8 | 1 | 5 | nt |
| 2 | CD8+HYA2 | 24 | 3 | 1 | 1 | nt |
| 3 | CD8+HYA2 | 24 | 5 | 0 | 2 | nt |
| 4 | CD8+HYA2 | 27 | 10 | 0 | 4 | nt |
| 5 | CD8+HYA2 | 47 | 11 | 3 | 6 | Negative |
| CD8+HYB7 | 12 | 1 | 0 | 0 | ||
| 6 | CD8+HYA2 | 25 | 8 | 3 | 7 | nt |
| 7 | CD8+HYA2CD45RO+ | 27 | 1 | 0 | 1 | Monocytes |
| CD8+HYA2CD45RO− | 48 | 3 | 1 | 1 | ||
| 8 | CD8+HYA2 | 8 | 0 | na | na | nt |
| 9 | CD8+HYA2CD45RO+ | 64 | 1 | 0 | 1 | mDC, B cell |
| 10 | CD8+HYA2 | 99 | 2 | 1 | 0 | B cell |
| CD8+HYB7 | 95 | 9 | 0 | 1 | ||
| 11 | CD8+HYA2 | 31 | 1 | 0 | 1 | Negative |
| CD8+HYA2CD45RO+ | 3 | 0 | na | na | ||
| 12 | CD8+HYA2HYB7HYB60 | 80 | HYA2: 2 | 0 | 2 | nt |
| HYB7: 1 | 1 | 1 | ||||
| HYB60: 0 | na | na | ||||
| 13 | nt | na | na | na | na | Negative |
| 14 | nt | na | na | na | na | mDC |
| 15 | nt | na | na | na | na | mDC |
| 16 | nt | na | na | na | na | Monocytes |
| Total, n = | 12 | 694 | 66 of 694 tested | 11 | 33 | 6 of 9 tested |
| 17 | CD8+HYA2 | 5 | 2 | 1 | 2 | nt |
| 18 | CD8+HYA2 | 25 | 5 | 1 | 1 | nt |
| 19 | CD8+HYA2 | 81 | 1 | 0 | 0 | nt |
| 20 | CD8+HYA2 | 234 | 13 | 2 | 3 | Negative |
| 21 | CD8+HYB7 | 70 | 17 | 2 | 3 | nt |
| 22 | nt | na | na | na | na | Negative |
| 23 | nt | na | na | na | na | Negative |
| Total, n = | 5 | 415 | 38 of 415 tested | 6 | 9 | 0 of 3 tested |
nt indicates not tested; mDC, myeloid dendritic cell; and na, not applicable.
Defined as at least 25% of specific lysis.