Secondary screening of 8 compounds identified from the high-throughput screen of ∼ 175 000 compounds
| No. . | Chemical name . | Library . | Concentration tested . | Percent inhibition of: . | |||
|---|---|---|---|---|---|---|---|
| Thrombin binding to polyP . | Kallikrein binding to polyP . | Free thrombin activity . | Free kallikrein activity . | ||||
| A | N-benzyl-2,2,6,6-tetramethyl-4-piperidinamine dihydrochloride | Chembridge | 3 μg/mL | 0 | 0 | 36.1 | NT |
| B | [(6-nitro-1,3-benzodioxol-5-yl)methylene]malononitrile | Chembridge | 10 μg/mL | 17.5 | 0 | 11.1 | 2.5 |
| C | 2-(allylthio)-1-(1,3-benzodioxol-5-ylcarbonyl)-1H-benzimidazole | Chembridge | 10 μg/mL | 95.1 | 81.4 | 99.6 | 97.4 |
| D | N-(2,4-dichlorophenyl)-N′-{5-[(4-nitrophenoxy) methyl]-1,3,4-thiadiazol-2-yl}urea | Chembridge | 10 μg/mL | 0 | 0 | 5.3 | 1.5 |
| E | 1-(3,4-dimethoxybenzoyl)-3-(2-furyl)-1H-1,2,4-triazol-5-amine | Chembridge | 10 μg/mL | 98.6 | 18.8 | 98.9 | 39.3 |
| F | 5-(benzylthio)-1-butyryl-3-phenyl-1H-1,2,4-triazole | Chembridge | 10 μg/mL | 92.8 | 9.6 | 99.5 | 12.3 |
| G | 2-(3-nitrophenyl)-3,1-benzoxazin-4(4H)-one | NCI Open Plate Set | 2μM | 2.0 | 0 | 3.8 | 0.6 |
| H | Surfen (bis-2-methyl-4-amino-quinolyl-6-carbamide) | NCI Diversity Set | 2μM | 95.6 | 55.3 | 32.2 | NT |
| No. . | Chemical name . | Library . | Concentration tested . | Percent inhibition of: . | |||
|---|---|---|---|---|---|---|---|
| Thrombin binding to polyP . | Kallikrein binding to polyP . | Free thrombin activity . | Free kallikrein activity . | ||||
| A | N-benzyl-2,2,6,6-tetramethyl-4-piperidinamine dihydrochloride | Chembridge | 3 μg/mL | 0 | 0 | 36.1 | NT |
| B | [(6-nitro-1,3-benzodioxol-5-yl)methylene]malononitrile | Chembridge | 10 μg/mL | 17.5 | 0 | 11.1 | 2.5 |
| C | 2-(allylthio)-1-(1,3-benzodioxol-5-ylcarbonyl)-1H-benzimidazole | Chembridge | 10 μg/mL | 95.1 | 81.4 | 99.6 | 97.4 |
| D | N-(2,4-dichlorophenyl)-N′-{5-[(4-nitrophenoxy) methyl]-1,3,4-thiadiazol-2-yl}urea | Chembridge | 10 μg/mL | 0 | 0 | 5.3 | 1.5 |
| E | 1-(3,4-dimethoxybenzoyl)-3-(2-furyl)-1H-1,2,4-triazol-5-amine | Chembridge | 10 μg/mL | 98.6 | 18.8 | 98.9 | 39.3 |
| F | 5-(benzylthio)-1-butyryl-3-phenyl-1H-1,2,4-triazole | Chembridge | 10 μg/mL | 92.8 | 9.6 | 99.5 | 12.3 |
| G | 2-(3-nitrophenyl)-3,1-benzoxazin-4(4H)-one | NCI Open Plate Set | 2μM | 2.0 | 0 | 3.8 | 0.6 |
| H | Surfen (bis-2-methyl-4-amino-quinolyl-6-carbamide) | NCI Diversity Set | 2μM | 95.6 | 55.3 | 32.2 | NT |
NT indicates not tested.