Enzyme kinetics
| . | Ap3A hydrolysis by NPP4 . | Ap4A hydrolysis by NPP4 . | ||
|---|---|---|---|---|
| Value . | SE . | Value . | SE . | |
| kcat, s−1NPP4−1 | 4.2 | ± 0.4 | 0.78 | ± 0.01 |
| KM, μM | 843.1 | ± 132 | 209.9 | ± 3 |
| kcat/KM | 5.0 × 103 | 3.7 × 103 | ||
| . | Ap3A hydrolysis by NPP4 . | Ap4A hydrolysis by NPP4 . | ||
|---|---|---|---|---|
| Value . | SE . | Value . | SE . | |
| kcat, s−1NPP4−1 | 4.2 | ± 0.4 | 0.78 | ± 0.01 |
| KM, μM | 843.1 | ± 132 | 209.9 | ± 3 |
| kcat/KM | 5.0 × 103 | 3.7 × 103 | ||
NPP4 indicates nucleotide pyrophosphatase/phosphodiesterase-4.