Results of RT-qPCR of frozen/thawed ovarian cortex
| Patient . | Fusion transcript, Ct . | Control transcript, Ct . | RNA concentration at extraction, ng/μL . | Amount of the ovary evaluated by RT-qPCR, % of 1 ovary . | Result . |
|---|---|---|---|---|---|
| Results before transplantation to nude mice | |||||
| 2 | 37.4 | 24 | 264 | 3 | Positive |
| 38.1 | 25 | 64 | 6 | ||
| 4 | 38.4 | 24 | 175 | 3.5 | Positive |
| Undetectable | 27 | 28 | 7 | ||
| 6 | 34.3 | 23 | 78 | 7 | Positive |
| Undetectable | 23 | 78 | |||
| 7 | Undetectable | 24 | 60 | 2.5 | Positive |
| 38.2 | 21 | 219 | 5 | ||
| 9 | Undetectable | 23 | 387 | 3 | Negative |
| 12 | Undetectable | 23 | 20 | 5 | Negative |
| Undetectable | 26 | 23 | 10 | ||
| 15 | Undetectable | 29 | 9 | 2 | Negative |
| Undetectable | 23 | 89 | 4 | ||
| Results after transplantation to nude mice | |||||
| 2 | Not performed | Not performed | Not performed | None left | NA |
| 4 | Undetectable | 26 | 12 | 3 | Negative |
| 6 | Undetectable | 27 | 10 | 7 | Negative |
| Undetectable | 28 | 10 | |||
| 7 | Undetectable | 24 | 18 | 2.5 | Negative |
| Undetectable | 23 | 36 | |||
| 9 | Undetectable | Undetectable | 1 | 1.5 | NA |
| 12 | Undetectable | 26 | 10 | 5 | Negative |
| Undetectable | 27 | 3 | |||
| 15 | Undetectable | Undetectable | 18 | 2 | Negative |
| Undetectable | 24 | 35 |
| Patient . | Fusion transcript, Ct . | Control transcript, Ct . | RNA concentration at extraction, ng/μL . | Amount of the ovary evaluated by RT-qPCR, % of 1 ovary . | Result . |
|---|---|---|---|---|---|
| Results before transplantation to nude mice | |||||
| 2 | 37.4 | 24 | 264 | 3 | Positive |
| 38.1 | 25 | 64 | 6 | ||
| 4 | 38.4 | 24 | 175 | 3.5 | Positive |
| Undetectable | 27 | 28 | 7 | ||
| 6 | 34.3 | 23 | 78 | 7 | Positive |
| Undetectable | 23 | 78 | |||
| 7 | Undetectable | 24 | 60 | 2.5 | Positive |
| 38.2 | 21 | 219 | 5 | ||
| 9 | Undetectable | 23 | 387 | 3 | Negative |
| 12 | Undetectable | 23 | 20 | 5 | Negative |
| Undetectable | 26 | 23 | 10 | ||
| 15 | Undetectable | 29 | 9 | 2 | Negative |
| Undetectable | 23 | 89 | 4 | ||
| Results after transplantation to nude mice | |||||
| 2 | Not performed | Not performed | Not performed | None left | NA |
| 4 | Undetectable | 26 | 12 | 3 | Negative |
| 6 | Undetectable | 27 | 10 | 7 | Negative |
| Undetectable | 28 | 10 | |||
| 7 | Undetectable | 24 | 18 | 2.5 | Negative |
| Undetectable | 23 | 36 | |||
| 9 | Undetectable | Undetectable | 1 | 1.5 | NA |
| 12 | Undetectable | 26 | 10 | 5 | Negative |
| Undetectable | 27 | 3 | |||
| 15 | Undetectable | Undetectable | 18 | 2 | Negative |
| Undetectable | 24 | 35 |
Ct indicates threshold cycle; RT-qPCR, reverse transcription–quantitative polymerase chain reaction; and NA, not available.