Patient characteristics for fresh skin biopsy analysis including aGVHD and cGVHD SCT patients
| Group . | Patient ID . | Disease . | Transplantation date . | Time after transplantation . | Conditioning . | Transplantation type . | Alem . | GVHD prophylaxis . | Stem cell source . | NIH classification (retrospective) . | Previous aGVHD . | Grade . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| aGVHD | Biopsy A | MDS | 06-May-09 | 4 wk | Cy/TBI | MUD | In vivo | CSA + MTX | PBSCs | Classic aGVHD | N/A | N/A |
| Biopsy B | MDS | 23-Apr-09 | 10 wk | Flu/Mel/Alem, no TBI | SIB | In vivo | CSA only | PBSCs | Classic aGVHD | N/A | N/A | |
| Biopsy C | CML | 25-Aug-09 | 4 wk | Flu/Bu/Alem | SIB | In vivo | CSA only | PBSCs | Classic aGVHD | N/A | N/A | |
| Biopsy D | MDS | 06-Oct-09 | 3 wk | Flu/Mel/Alem, no TBI | SIB | In vivo | CSA only | PBSCs | Classic aGVHD | N/A | N/A | |
| Biopsy E | MDS | 22-Sep-09 | 11 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Classic aGVHD | N/A | N/A | |
| Biopsy F | NHL | 20-Apr-10 | 11 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Classic aGVHD | N/A | N/A | |
| Biopsy G | B-ALL | 02-Jun-10 | 7 wk | Cy/TBI | SIB | None | CSA + MTX | PBSCs | Classic aGVHD | N/A | N/A | |
| Biopsy H | MDS | 29-Jun-10 | 3 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Classic aGVHD | N/A | N/A | |
| Biopsy I | CML | 12-Aug-10 | 6 wk | Flu/Bu/Alem | MUD | In vivo | CSA only | PBSCs | Classic aGVHD | N/A | N/A | |
| Biopsy J | AML | 16-Nov-10 | 4 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Classic aGVHD | N/A | N/A | |
| cGVHD | Biopsy K | MF | 28-Apr-09 | 33 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Late aGVHD | Skin | Grade I |
| Biopsy L | AML | 07-Apr-10 | 25 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Classic cGVHD | None | N/A | |
| Biopsy M | AML | 14-Apr-10 | 22 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Late aGVHD | Skin | Grade II | |
| Biopsy N | CLL | 20-Oct-09 | 47 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Classic cGVHD | None | N/A | |
| Biopsy O | AML | 27-Apr-10 | 23 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Classic cGVHD | None | N/A | |
| Biopsy P | MDS | 06-Oct-09 | 66 wk | Flu/Mel/Alem, no TBI | SIB | In vivo | CSA only | PBSCs | Classic cGVHD | Skin | Grade II | |
| Biopsy Q | AML | 17-Aug-10 | 24 wk | Flu/Mel/Alem, no TBI | SIB | In vivo | CSA only | PBSCs | Classic cGVHD | Gut | Not known | |
| Biopsy R | TPL | 22-Jun-10 | 78 wk | Cy/TBI | SIB | None | CSA + MTX | PBSCs | Late aGVHD | Gut | Grade II | |
| Biopsy S | CML | 05-Oct-10 | 42 wk | Cy/TBI/Alem | MUD | In vivo | CSA + MTX | PBSCs | Classic cGVHD | Skin | Grade I |
| Group . | Patient ID . | Disease . | Transplantation date . | Time after transplantation . | Conditioning . | Transplantation type . | Alem . | GVHD prophylaxis . | Stem cell source . | NIH classification (retrospective) . | Previous aGVHD . | Grade . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| aGVHD | Biopsy A | MDS | 06-May-09 | 4 wk | Cy/TBI | MUD | In vivo | CSA + MTX | PBSCs | Classic aGVHD | N/A | N/A |
| Biopsy B | MDS | 23-Apr-09 | 10 wk | Flu/Mel/Alem, no TBI | SIB | In vivo | CSA only | PBSCs | Classic aGVHD | N/A | N/A | |
| Biopsy C | CML | 25-Aug-09 | 4 wk | Flu/Bu/Alem | SIB | In vivo | CSA only | PBSCs | Classic aGVHD | N/A | N/A | |
| Biopsy D | MDS | 06-Oct-09 | 3 wk | Flu/Mel/Alem, no TBI | SIB | In vivo | CSA only | PBSCs | Classic aGVHD | N/A | N/A | |
| Biopsy E | MDS | 22-Sep-09 | 11 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Classic aGVHD | N/A | N/A | |
| Biopsy F | NHL | 20-Apr-10 | 11 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Classic aGVHD | N/A | N/A | |
| Biopsy G | B-ALL | 02-Jun-10 | 7 wk | Cy/TBI | SIB | None | CSA + MTX | PBSCs | Classic aGVHD | N/A | N/A | |
| Biopsy H | MDS | 29-Jun-10 | 3 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Classic aGVHD | N/A | N/A | |
| Biopsy I | CML | 12-Aug-10 | 6 wk | Flu/Bu/Alem | MUD | In vivo | CSA only | PBSCs | Classic aGVHD | N/A | N/A | |
| Biopsy J | AML | 16-Nov-10 | 4 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Classic aGVHD | N/A | N/A | |
| cGVHD | Biopsy K | MF | 28-Apr-09 | 33 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Late aGVHD | Skin | Grade I |
| Biopsy L | AML | 07-Apr-10 | 25 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Classic cGVHD | None | N/A | |
| Biopsy M | AML | 14-Apr-10 | 22 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Late aGVHD | Skin | Grade II | |
| Biopsy N | CLL | 20-Oct-09 | 47 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Classic cGVHD | None | N/A | |
| Biopsy O | AML | 27-Apr-10 | 23 wk | Flu/Mel/Alem, no TBI | MUD | In vivo | CSA only | PBSCs | Classic cGVHD | None | N/A | |
| Biopsy P | MDS | 06-Oct-09 | 66 wk | Flu/Mel/Alem, no TBI | SIB | In vivo | CSA only | PBSCs | Classic cGVHD | Skin | Grade II | |
| Biopsy Q | AML | 17-Aug-10 | 24 wk | Flu/Mel/Alem, no TBI | SIB | In vivo | CSA only | PBSCs | Classic cGVHD | Gut | Not known | |
| Biopsy R | TPL | 22-Jun-10 | 78 wk | Cy/TBI | SIB | None | CSA + MTX | PBSCs | Late aGVHD | Gut | Grade II | |
| Biopsy S | CML | 05-Oct-10 | 42 wk | Cy/TBI/Alem | MUD | In vivo | CSA + MTX | PBSCs | Classic cGVHD | Skin | Grade I |
Shown are the clinical features of fresh skin biopsies received from patients with aGVHD and cGVHD after SCT.
MDS, myelodysplastic syndrome; CML, chronic myeloid leukemia; NHL, non-Hodgkin lymphoma; B-ALL, B-cell acute lymphoblastic leukemia; AML, acute myeloid leukemia; CLL, chronic lymphocytic leukemia; TPL, T-prolymphocytic leukemia; Cy, cyclophosphamide; TBI, total body irradiation; Flu, fludarabine; Mel, melphalan; Alem, alemtuzumab; Bu, busulfan; MUD, matched unrelated donor; SIB, sibling donor; MTX, methotrexate; MF, myelofibrosis; and PBSCs, peripheral blood stem cells.