Gene expression assessed by threshold cycle values in qRT-PCR
| . | 3T3 cells . | 3T3-L1 cells . | mBMMNCs . |
|---|---|---|---|
| GATA1 | ND | ND | 8.79 ± 0.31 |
| GATA2 | 8.81 ± 0.06 | 8.08 ± 0.07 | 3.88 ± 0.37 |
| p45NF-E2 | ND | 17.91 ± 0.14 | 3.96 ± 0.01 |
| FOG1 | 9.52 ± 0.07 | 8.04 ± 0.08 | 12.00 ± 0.12 |
| Fli1 | 14.00 ± 0.2 | 13.21 ± 0.18 | 4.10 ± 0.01 |
| RUNX1 | 7.62 ± 0.07 | 8.50 ± 0.16 | 6.52 ± 0.00 |
| CEBPα | ND | 11.10 ± 0.27 | 7.04 ± 0.01 |
| PPAR-γ | 16.21 ± 0.01 | 7.14 ± 0.29 | 10.47 ± 0.61 |
| . | 3T3 cells . | 3T3-L1 cells . | mBMMNCs . |
|---|---|---|---|
| GATA1 | ND | ND | 8.79 ± 0.31 |
| GATA2 | 8.81 ± 0.06 | 8.08 ± 0.07 | 3.88 ± 0.37 |
| p45NF-E2 | ND | 17.91 ± 0.14 | 3.96 ± 0.01 |
| FOG1 | 9.52 ± 0.07 | 8.04 ± 0.08 | 12.00 ± 0.12 |
| Fli1 | 14.00 ± 0.2 | 13.21 ± 0.18 | 4.10 ± 0.01 |
| RUNX1 | 7.62 ± 0.07 | 8.50 ± 0.16 | 6.52 ± 0.00 |
| CEBPα | ND | 11.10 ± 0.27 | 7.04 ± 0.01 |
| PPAR-γ | 16.21 ± 0.01 | 7.14 ± 0.29 | 10.47 ± 0.61 |
Values (mean ± SD) are the threshold cycles of target normalized with GAPDH levels. mBMMNCs were used as a control, because expression of GATA 1 was not observed in both 3T3 cells and 3T3-L1 cells.
ND indicates not detected, that is, threshold more than 40 cycles.