List of antibodies used in dual immunofluorescence experiments
| . | Targets . | Clone . | Dilution . |
|---|---|---|---|
| Monoclonal antibodies | |||
| Anti-CD1c | B cells in particular MZ analog B cells | C4445 | 1/20 |
| FITC–anti-CD27 | T cells, plasma cells and B cells in particular MZ analog and memory B cells | M-T271 | 1/20 |
| FITC–anti-CD3 | T cells | UCHT1 | 1/50 |
| FITC–anti-IgM | Plasma cells and B cells | G20-127 | 1/20 |
| FITC–anti-IgD | B cells in particular mantle and memory B cells | IA6-2 | 1/20 |
| Anti-GPIIb/IIIa | Platelet glycoprotein IIb/IIIa: a main target of autoantibody in ITP | 2B3A | 1/100 |
| FITC–anti-Ki67 | Proliferating cells | MiB-1 | 1/20 |
| FITC–anti-CD35 | B cells and FDCs | E11 | 1/20 |
| Polyclonal antibodies | None | ||
| Rabbit anti–human CD20 | B cells | pAbs | 1/200 |
| Rabbit anti–human IgM | Plasma cells and B cells | pAbs | 1/400 |
| FITC-goat anti–mouse Ig | pAbs | 1/30 | |
| FITC-donkey anti–rabbit Ig | pAbs | 1/100 | |
| RRX-donkey anti–rabbit Ig | pAbs | 1/100 | |
| RRX-donkey anti–mouse Ig | pAbs | 1/100 |
| . | Targets . | Clone . | Dilution . |
|---|---|---|---|
| Monoclonal antibodies | |||
| Anti-CD1c | B cells in particular MZ analog B cells | C4445 | 1/20 |
| FITC–anti-CD27 | T cells, plasma cells and B cells in particular MZ analog and memory B cells | M-T271 | 1/20 |
| FITC–anti-CD3 | T cells | UCHT1 | 1/50 |
| FITC–anti-IgM | Plasma cells and B cells | G20-127 | 1/20 |
| FITC–anti-IgD | B cells in particular mantle and memory B cells | IA6-2 | 1/20 |
| Anti-GPIIb/IIIa | Platelet glycoprotein IIb/IIIa: a main target of autoantibody in ITP | 2B3A | 1/100 |
| FITC–anti-Ki67 | Proliferating cells | MiB-1 | 1/20 |
| FITC–anti-CD35 | B cells and FDCs | E11 | 1/20 |
| Polyclonal antibodies | None | ||
| Rabbit anti–human CD20 | B cells | pAbs | 1/200 |
| Rabbit anti–human IgM | Plasma cells and B cells | pAbs | 1/400 |
| FITC-goat anti–mouse Ig | pAbs | 1/30 | |
| FITC-donkey anti–rabbit Ig | pAbs | 1/100 | |
| RRX-donkey anti–rabbit Ig | pAbs | 1/100 | |
| RRX-donkey anti–mouse Ig | pAbs | 1/100 |
MZ indicates marginal zone; ITP, immune thrombocytopenia; and FDC, follicular dendritic cell.