Table 1 BM and WAT cellularity in mice... | American Society of Hematology

Table 1

BM and WAT cellularity in mice repopulated with WAT-KLS or BM-KLS

	Total cell no., ×10⁶		Percentage of CD45⁺ cells, % of total cells		Percentage of CD45.1⁺ cells, % of CD45⁺ cells		Absolute no. of KLS cells, ×10⁴
	BM	WAT	BM	WAT	BM	WAT	BM	WAT
WAT-KLS–repopulated mice	9.69 ± 1.71	1.36 ± 0.14	84.86 ± 1.80	64.66 ± 4.27	0.94 ± 0.2	33.6 ± 4.8	2.5 ± 0.4	2.4 ± 0.6
BM-KLS–repopulated mice	7.16 ± 1.03	1.32 ± 0.24	84.39 ± 1.66	43.98 ± 5.39*	24 ± 6.6†	30.75 ± 5.7	2.1 ± 0.7	0.48 ± 0.01‡

	Total cell no., ×10⁶		Percentage of CD45⁺ cells, % of total cells		Percentage of CD45.1⁺ cells, % of CD45⁺ cells		Absolute no. of KLS cells, ×10⁴
	BM	WAT	BM	WAT	BM	WAT	BM	WAT
WAT-KLS–repopulated mice	9.69 ± 1.71	1.36 ± 0.14	84.86 ± 1.80	64.66 ± 4.27	0.94 ± 0.2	33.6 ± 4.8	2.5 ± 0.4	2.4 ± 0.6
BM-KLS–repopulated mice	7.16 ± 1.03	1.32 ± 0.24	84.39 ± 1.66	43.98 ± 5.39*	24 ± 6.6†	30.75 ± 5.7	2.1 ± 0.7	0.48 ± 0.01‡

Lethally irradiated CD45.2 mice were intravenously injected with 10³ CD45.1 WAT-KLS or BM-KLS cells together with 2.10⁵ CD45.2 total BM cells. Four months after injection, BM and WAT were removed and total BM and SVF cells were prepared as described in “Isolation of WAT, spleen, thymus, ganglion, and BM cells.” The total cell number was quantified by cell counting and CD45⁺ and KLS cell numbers were estimated by flow cytometry. Results are expressed as means ± SEM of 7 independent experiments.

P < .001.

†

P < .0001 in WAT-KLS– versus BM-KLS–repopulated mice.

‡

P < .05.

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