Inhibition of cell-to-cell transfer by mAbs and MoDC maturation
| Monoclonal IgG . | HIV-1BaL . | |||
|---|---|---|---|---|
| IC70, μg/mL . | Statistical analysis . | MoDC maturation . | ||
| T cells . | MoDCs . | |||
| NAbs | ||||
| Anti-gp120 | ||||
| 2G12 | 4 ± 3* | 19 ± 13* | P = .0021 (n = 10) | − (n = 5) |
| b12 | 11 ± 5* | 48 ± 23‡ | P < .0001 (n = 14) | ++ (n = 7) |
| VRC01 | 7 ± 5* | 98 ± 56§ | P = .0159 (n = 5) | + (n = 4) |
| VRC03 | 9 ± 5* | 91 ± 57§ | P = .0286 (n = 4) | + (n = 3) |
| Anti-gp41 | ||||
| 2F5 | 27 ± 18† | 13 ± 9* | P = .0262 (n = 12) | ++ (n = 6) |
| 4 E10 | 47 ± 28‡ | 19 ± 11* | P = .0037 (n = 15) | +++ (n = 9) |
| NNAbs | ||||
| 4B3 | > 100¶ | > 100¶ | P = .0159 (n = 6) | − (n = 5) |
| 246-D | > 100¶ | 75 ± 25§ | P < .0001 (n = 9) | ++ (n = 6) |
| 5F3 | > 100¶ | > 100¶ | P = .4000 (n = 3) | + (n = 2) |
| 1570-D | > 100¶ | > 100¶ | P = .8857 (n = 4) | − (n = 2) |
| Monoclonal IgG . | HIV-1BaL . | |||
|---|---|---|---|---|
| IC70, μg/mL . | Statistical analysis . | MoDC maturation . | ||
| T cells . | MoDCs . | |||
| NAbs | ||||
| Anti-gp120 | ||||
| 2G12 | 4 ± 3* | 19 ± 13* | P = .0021 (n = 10) | − (n = 5) |
| b12 | 11 ± 5* | 48 ± 23‡ | P < .0001 (n = 14) | ++ (n = 7) |
| VRC01 | 7 ± 5* | 98 ± 56§ | P = .0159 (n = 5) | + (n = 4) |
| VRC03 | 9 ± 5* | 91 ± 57§ | P = .0286 (n = 4) | + (n = 3) |
| Anti-gp41 | ||||
| 2F5 | 27 ± 18† | 13 ± 9* | P = .0262 (n = 12) | ++ (n = 6) |
| 4 E10 | 47 ± 28‡ | 19 ± 11* | P = .0037 (n = 15) | +++ (n = 9) |
| NNAbs | ||||
| 4B3 | > 100¶ | > 100¶ | P = .0159 (n = 6) | − (n = 5) |
| 246-D | > 100¶ | 75 ± 25§ | P < .0001 (n = 9) | ++ (n = 6) |
| 5F3 | > 100¶ | > 100¶ | P = .4000 (n = 3) | + (n = 2) |
| 1570-D | > 100¶ | > 100¶ | P = .8857 (n = 4) | − (n = 2) |
The inhibitory activity of mAbs added at different concentrations was analyzed in the HIV-1–transfer assay. The concentration of Ab resulting in IC70 was calculated in parallel for HIV-1 transfer to CD4 T lymphocytes and for HIV-1 replication in MoDCs. IC70 values are expressed as means ± SD. Statistical analysis of pairwise comparisons was based on t tests (Mann-Whitney test), with P < .05 considered significant. MoDC maturation is presented as the fold increase (F.I.), calculated as the percentage of CD83+ DC-SIGN+ MoDCs in the presence of Abs divided by the percentage of CD83+ DC-SIGN+ control MoDCs in the absence of Abs.
+++ indicates > 3 F.I.; ++, 2.1-3 F.I.; +, 1.2-2 F.I.; and −, < 1.2 F.I.
< 20 μg/mL.
20-40 μg/mL.
40-80 μg/mL.
80-100 μg/mL.
> 100 μg/mL.