List of primers used for generation of huVWF mutants
| Primer names . | Nucleotide sequences . |
|---|---|
| huVWF H1326R S | 5′-CACGACGGCTCACGGGCTTACATCGGG-3′ |
| huVWF H1326R AS | 5′-CCCGATGTAAGCCCGTGAGCCGTCGTG-3′ |
| huVWF Q1391P S | 5′-CAGGAGCCCCCACGGATGTCC-3′ |
| huVWF Q1391P AS | 5′-GGACATCCGTGGGGGCTCCTG-3′ |
| huVWFmu (1306-1314) S | 5′-AGGTTACACATCTCTCAGAAGCGCATCCGTGTGGCCGTGGTG-3′ |
| huVWFmu (1306-1314) AS | 5′-GATGCGCTTCTGTGAGATGTGTAACCTCTCCATCATGTCGACCACA-3′ |
| huVWFmu (1326-1333) S | 5′-CGCGCATATCTAGAACTCAAGGCCCGTAAGCGACCGTCAGAG-3′ |
| huVWFmu (1326-1333) AS | 5′-GGCCTTGAGTTCTAGATATGCGCGGGAGCCATCGTGGTACTC-3′ |
| huVWFmu (1370-1385) S | 5′-CGCCGTGAAGCCTCCCATATCACTCTACTA-3′ |
| huVWFmu (1370-1385) AS | 5′-AGTGATATGGGAGGCTTCAGGGCGATCAAT-3′ |
| huVWFmu (1326-1333; 1370-1385) S | 5′-CGCCGTGAAGCCTCCCATATCACTCTACTA-3′ |
| huVWFmu (1326-1333; 1370-1385) AS | 5′-AGTGATATGGGAGGCTTCAGGGCGATCAAT-3′ |
| huVWFmuA1 S | 5′-CCTCCCACAGATGCCCCGGTCAGCTCTAC-3′ |
| huVWFmuA1 AS | 5′-CAGAACCATGGACTTCCGTTT-3′ |
| Primer names . | Nucleotide sequences . |
|---|---|
| huVWF H1326R S | 5′-CACGACGGCTCACGGGCTTACATCGGG-3′ |
| huVWF H1326R AS | 5′-CCCGATGTAAGCCCGTGAGCCGTCGTG-3′ |
| huVWF Q1391P S | 5′-CAGGAGCCCCCACGGATGTCC-3′ |
| huVWF Q1391P AS | 5′-GGACATCCGTGGGGGCTCCTG-3′ |
| huVWFmu (1306-1314) S | 5′-AGGTTACACATCTCTCAGAAGCGCATCCGTGTGGCCGTGGTG-3′ |
| huVWFmu (1306-1314) AS | 5′-GATGCGCTTCTGTGAGATGTGTAACCTCTCCATCATGTCGACCACA-3′ |
| huVWFmu (1326-1333) S | 5′-CGCGCATATCTAGAACTCAAGGCCCGTAAGCGACCGTCAGAG-3′ |
| huVWFmu (1326-1333) AS | 5′-GGCCTTGAGTTCTAGATATGCGCGGGAGCCATCGTGGTACTC-3′ |
| huVWFmu (1370-1385) S | 5′-CGCCGTGAAGCCTCCCATATCACTCTACTA-3′ |
| huVWFmu (1370-1385) AS | 5′-AGTGATATGGGAGGCTTCAGGGCGATCAAT-3′ |
| huVWFmu (1326-1333; 1370-1385) S | 5′-CGCCGTGAAGCCTCCCATATCACTCTACTA-3′ |
| huVWFmu (1326-1333; 1370-1385) AS | 5′-AGTGATATGGGAGGCTTCAGGGCGATCAAT-3′ |
| huVWFmuA1 S | 5′-CCTCCCACAGATGCCCCGGTCAGCTCTAC-3′ |
| huVWFmuA1 AS | 5′-CAGAACCATGGACTTCCGTTT-3′ |
Names of the primers are composed of the name of the VWF constructs; S and AS represent forward and reverse primers, respectively. For all constructs, the pCDNA6-huVWF plasmid containing the full-length cDNA of human VWF (huVWF)17 was used as a template, except for constructs huVWFmu (1326-1333; 1370-1385) and huVWFmuA1 where pCDNA6-huVWFmu (1326-1333) and pCDNA6-muVwf plasmids, respectively were used as templates.