Comparison of the average protein-density changes and the frequency of protein-expression changes in leukemic samples cultured alone or cocultured with MS-5 cells
| Protein* . | Average density change† . | P‡ . | ↑Expression (n)§ . | ↓Expression (n)‖ . | P¶ . |
|---|---|---|---|---|---|
| pAKT (Thr308) | +0.880 | .001 | 18 | 2 | < .001 |
| pβ-catenin (Ser33/37/Thr41) | −0.830 | .002 | 3 | 15 | .035 |
| pERK (Thr202/Tyr204) | +0.660 | .002 | 14 | 3 | .039 |
| mTOR | NS | NS | 17 | 0 | < .001 |
| PRAS40 | NS | NS | 0 | 13 | < .001 |
| pSTAT3 (Ser727) | +0.298 | .013 | 12 | 2 | .039 |
| Protein* . | Average density change† . | P‡ . | ↑Expression (n)§ . | ↓Expression (n)‖ . | P¶ . |
|---|---|---|---|---|---|
| pAKT (Thr308) | +0.880 | .001 | 18 | 2 | < .001 |
| pβ-catenin (Ser33/37/Thr41) | −0.830 | .002 | 3 | 15 | .035 |
| pERK (Thr202/Tyr204) | +0.660 | .002 | 14 | 3 | .039 |
| mTOR | NS | NS | 17 | 0 | < .001 |
| PRAS40 | NS | NS | 0 | 13 | < .001 |
| pSTAT3 (Ser727) | +0.298 | .013 | 12 | 2 | .039 |
NS indicates not significant.
Proteins with significant density changes and/or frequent alteration in response to coculture.
Changes in the average protein density in all samples (see “Reverse-phase protein array” for details). The positive sign represents an increase, whereas the negative sign represents a decrease in the average protein density.
Adjusted P values (see “Reverse-phase protein array”). The cutoff P value for significance was ≤ .05.
Number of primary samples with up-regulation of expression of the indicated protein (please see “Reverse-phase protein array” for statistical analysis).
Number of primary samples with down-regulation of expression of the indicated protein.
Comparison of the numbers of samples with detectable up-regulation and down-regulation of protein expression using conditional binomial tests (P value) to confirm that alteration was heavily skewed (P ≤ .05).