Differential hematopoietic cell counts on BM sections in control and TPO-treated chimeric mice
| . | WT > WT saline . | WT > Sparc−/− saline . | Sparc−/− > WT saline . | Sparc−/− > Sparc−/− saline . | WT > WT TPO . | WT > Sparc−/− TPO . | Sparc−/− > WT TPO . | Sparc−/− > Sparc−/− TPO . | P value of treatment . | P value of host SPARC genotype . |
|---|---|---|---|---|---|---|---|---|---|---|
| Neutrophils | 89.6 ± 7.63 | 113.8 ± 13.0 | 114.0 ± 8.3 | 122.2 ± 5.4 | 138.7 ± 15.3 | 164.33 ± 19.3 | 156.2 ± 19.9 | 182 ± 8.13 | < .0001 | < .0001 |
| Eosinophils | 4.2 ± 1.8 | 3.0 ± 1.2 | 3.4 ± 1.1 | 4 ± 1.2 | 12.1 ± 3.2 | 10.5 ± 1.4 | 13.1 ± 2.9 | 12.3 ± 2.0 | < .0001 | .2443 |
| Immature myeloid cells | 31.6 ± 7.2 | 37.6 ± 9.8 | 41 ± 7.1 | 46.2 ± 4.1 | 46.3 ± 4.7 | 56.2 ± 5.5 | 44.3 ± 5.4 | 61.3 ± 6.6 | < .0001 | .0004 |
| Erythroid cells | 66.8 ± 10.3 | 69.2 ± 7.2 | 69.2 ± 5.11 | 68.8 ± 6.1 | 52.7 ± 9.5 | 40 ± 4.9 | 38.7 ± 7.5 | 33.5 ± 10.1 | < .0001 | .1241 |
| Megakaryocytes | 11.6 ± 3.6 | 11.6 ± 3.2 | 12.8 ± 2.4 | 14 ± 1.6 | 49 ± 2.3 | 62.3 ± 3.4 | 53 ± 13.0 | 74.8 ± 5.6 | < .0001 | < .0001 |
| Lymphoid cells | 44.6 ± 10.2 | 52.0 ± 13.8 | 54.6 ± 10.3 | 54.8 ± 8.7 | 46.8 ± 5.0 | 54.5 ± 4.9 | 34.5 ± 7.4 | 28 ± 7.6 | .0011 | .0405 |
| . | WT > WT saline . | WT > Sparc−/− saline . | Sparc−/− > WT saline . | Sparc−/− > Sparc−/− saline . | WT > WT TPO . | WT > Sparc−/− TPO . | Sparc−/− > WT TPO . | Sparc−/− > Sparc−/− TPO . | P value of treatment . | P value of host SPARC genotype . |
|---|---|---|---|---|---|---|---|---|---|---|
| Neutrophils | 89.6 ± 7.63 | 113.8 ± 13.0 | 114.0 ± 8.3 | 122.2 ± 5.4 | 138.7 ± 15.3 | 164.33 ± 19.3 | 156.2 ± 19.9 | 182 ± 8.13 | < .0001 | < .0001 |
| Eosinophils | 4.2 ± 1.8 | 3.0 ± 1.2 | 3.4 ± 1.1 | 4 ± 1.2 | 12.1 ± 3.2 | 10.5 ± 1.4 | 13.1 ± 2.9 | 12.3 ± 2.0 | < .0001 | .2443 |
| Immature myeloid cells | 31.6 ± 7.2 | 37.6 ± 9.8 | 41 ± 7.1 | 46.2 ± 4.1 | 46.3 ± 4.7 | 56.2 ± 5.5 | 44.3 ± 5.4 | 61.3 ± 6.6 | < .0001 | .0004 |
| Erythroid cells | 66.8 ± 10.3 | 69.2 ± 7.2 | 69.2 ± 5.11 | 68.8 ± 6.1 | 52.7 ± 9.5 | 40 ± 4.9 | 38.7 ± 7.5 | 33.5 ± 10.1 | < .0001 | .1241 |
| Megakaryocytes | 11.6 ± 3.6 | 11.6 ± 3.2 | 12.8 ± 2.4 | 14 ± 1.6 | 49 ± 2.3 | 62.3 ± 3.4 | 53 ± 13.0 | 74.8 ± 5.6 | < .0001 | < .0001 |
| Lymphoid cells | 44.6 ± 10.2 | 52.0 ± 13.8 | 54.6 ± 10.3 | 54.8 ± 8.7 | 46.8 ± 5.0 | 54.5 ± 4.9 | 34.5 ± 7.4 | 28 ± 7.6 | .0011 | .0405 |
Data have been analyzed with a 2-way ANOVA. P value expresses how the treatment (TPO) and the Sparc host genotype affect the variables.