Analysis of 3′HS1 and 3′HS2 function in transgenic mice
| Construct . | Line . | Copy no. . | huβ-mRNA/mα-mRNA . | huβ-mRNA/copy* . | Uniform, variegated . | ||||
|---|---|---|---|---|---|---|---|---|---|
| β-globin | A | 1 | 0.001 + 0.0005† | 0.001 | ND | ||||
| B | 2 | 0.0025 + 0.002† | 0.001 | ND | |||||
| C | 1 | 0.002 + 0.001† | 0.002 | ND | |||||
| D | 1 | 0.001 + 0.0001† | 0.001 | ND | |||||
| E | 3 | 0.000 + 0.0003† | 0.000 | ND | |||||
| F | 1 | 0.066 + 0.012 | 0.066 | U | |||||
| G | 1 | 0.001 + 0.0001† | 0.001 | ND | |||||
| H | 2 | 0.002 + 0.001† | 0.001 | ND | |||||
| I | 1 | 0.001 + 0.0003† | 0.001 | ND | |||||
| J | 1 | 0.002 + 0.0005† | 0.002 | ND | |||||
| Mean: 0.008 + 0.02 | |||||||||
| 3′HS1-β-globin-3′HS1 | A | 10 | 2.47 + 0.46 | 0.247 | U | ||||
| B | 1 | 0.17 + 0.04 | 0.17 | U | |||||
| C | 4 | 0.22 + 0.024 | 0.06 | V | |||||
| D | 3 | 0.23 + 0.012 | 0.078 | V | |||||
| E | 3 | 0.37 + 0.02 | 0.12 | U | |||||
| F | 4 | 2.26 + 0.17 | 0.565 | V | |||||
| Mean: 0.21 + 0.19 P = 0.009‡ | |||||||||
| 3′HS2-β-globin-3′HS2 | A | 1 | 0.033 + 0.005 | 0.033 | U | ||||
| B | 1 | 0.011 + 0.002 | 0.011 | V | |||||
| C | 1 | 0.002 + 0.001† | 0.002 | ND | |||||
| D | 2 | 0.028 + 0.01 | 0.014 | U | |||||
| E | 2 | 0.018 + 0.002 | 0.009 | ND | |||||
| F | 1 | 0.047 + 0.012 | 0.047 | V | |||||
| Mean: 0.02 + 0.02 P = 0.186‡ | |||||||||
| 3′HS1 + 2-β-globin-3′HS1 + 2 | A | 4 | 0.127 + 0.034 | 0.032 | U | ||||
| B | 5 | 0.05 + 0.008 | 0.01 | ND | |||||
| C | 2 | 0.083 + 0.012 | 0.042 | U | |||||
| D | 3 | 0.012 + 0.006 | 0.004 | ND | |||||
| E | 6 | 0.192 + 0.055 | 0.032 | U | |||||
| F | 1 | 0.053 + 0.013 | 0.053 | ND | |||||
| G | 1 | 0.037 + 0.013 | 0.037 | ND | |||||
| H | 3 | 0.147 + 0.062 | 0.049 | U | |||||
| I | 9 | 0.22 + 0.037 | 0.025 | U | |||||
| J | 5 | 0.13 + 0.016 | 0.026 | U | |||||
| Mean: 0.031 + 0.016 | |||||||||
| Construct . | Line . | Copy no. . | huβ-mRNA/mα-mRNA . | huβ-mRNA/copy* . | Uniform, variegated . | ||||
|---|---|---|---|---|---|---|---|---|---|
| β-globin | A | 1 | 0.001 + 0.0005† | 0.001 | ND | ||||
| B | 2 | 0.0025 + 0.002† | 0.001 | ND | |||||
| C | 1 | 0.002 + 0.001† | 0.002 | ND | |||||
| D | 1 | 0.001 + 0.0001† | 0.001 | ND | |||||
| E | 3 | 0.000 + 0.0003† | 0.000 | ND | |||||
| F | 1 | 0.066 + 0.012 | 0.066 | U | |||||
| G | 1 | 0.001 + 0.0001† | 0.001 | ND | |||||
| H | 2 | 0.002 + 0.001† | 0.001 | ND | |||||
| I | 1 | 0.001 + 0.0003† | 0.001 | ND | |||||
| J | 1 | 0.002 + 0.0005† | 0.002 | ND | |||||
| Mean: 0.008 + 0.02 | |||||||||
| 3′HS1-β-globin-3′HS1 | A | 10 | 2.47 + 0.46 | 0.247 | U | ||||
| B | 1 | 0.17 + 0.04 | 0.17 | U | |||||
| C | 4 | 0.22 + 0.024 | 0.06 | V | |||||
| D | 3 | 0.23 + 0.012 | 0.078 | V | |||||
| E | 3 | 0.37 + 0.02 | 0.12 | U | |||||
| F | 4 | 2.26 + 0.17 | 0.565 | V | |||||
| Mean: 0.21 + 0.19 P = 0.009‡ | |||||||||
| 3′HS2-β-globin-3′HS2 | A | 1 | 0.033 + 0.005 | 0.033 | U | ||||
| B | 1 | 0.011 + 0.002 | 0.011 | V | |||||
| C | 1 | 0.002 + 0.001† | 0.002 | ND | |||||
| D | 2 | 0.028 + 0.01 | 0.014 | U | |||||
| E | 2 | 0.018 + 0.002 | 0.009 | ND | |||||
| F | 1 | 0.047 + 0.012 | 0.047 | V | |||||
| Mean: 0.02 + 0.02 P = 0.186‡ | |||||||||
| 3′HS1 + 2-β-globin-3′HS1 + 2 | A | 4 | 0.127 + 0.034 | 0.032 | U | ||||
| B | 5 | 0.05 + 0.008 | 0.01 | ND | |||||
| C | 2 | 0.083 + 0.012 | 0.042 | U | |||||
| D | 3 | 0.012 + 0.006 | 0.004 | ND | |||||
| E | 6 | 0.192 + 0.055 | 0.032 | U | |||||
| F | 1 | 0.053 + 0.013 | 0.053 | ND | |||||
| G | 1 | 0.037 + 0.013 | 0.037 | ND | |||||
| H | 3 | 0.147 + 0.062 | 0.049 | U | |||||
| I | 9 | 0.22 + 0.037 | 0.025 | U | |||||
| J | 5 | 0.13 + 0.016 | 0.026 | U | |||||
| Mean: 0.031 + 0.016 | |||||||||
U indicates uniform expression (100% of cells express human β-globin); V, variegated expression (< 100% of cells express human β-globin); and ND, level of human β-globin protein was insufficient for analysis.
huβ-mRNA/copy = huβ-mRNA/mα-mRNA divided by copy number for each line.
mRNA levels below 0.005 are indistinguishable from background and are scored as negative.
Compared with 3′HS1 + 2-β-globin-3′HS1 + 2.