Table 1

Effects of A11 on the adhesive properties of thrombin-activated platelets to human monocytes

Concentration, μg/mLCell bound, %
< 5 attached platelets5-10 attached platelets> 10 attached platelets
A11    
    0 31 66 
    10 11 48 51 
    100 22 51 27 
    1000 29 57 14 
Concentration, μg/mLCell bound, %
< 5 attached platelets5-10 attached platelets> 10 attached platelets
A11    
    0 31 66 
    10 11 48 51 
    100 22 51 27 
    1000 29 57 14 

Platelets (2 × 108 cells/mL) were first activated by thrombin, and then incubated with various concentrations of A11 for 4 hours at 37°C. Twenty microliters of A11-treated activated platelets were added to an equal volume of human monocytes (2 × 106 cells/mL) and incubated at room temperature for 30 minutes followed by inspection and scoring of the monocytes with various numbers of attached platelets: monocytes with less than 5, between 5 and 10, and more than 10 attached platelets.

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