Summary of Ig sequence data from 9 EBV-transformed B-CLL cell lines
| IGHV . | IGKV/IGLV* . | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CLL ID . | Cell line . | Source . | Family . | Mutation, % . | CDR1 . | CDR2 . | CDR3 . | Family . | Mutation, % . | CDR1 . | CDR2 . | CDR3 . |
| CLL246 | B-CLL | PBMCs | 1-69 | 0.3 | GGTFSSYA | IIPILGIA | ARSDQNYDFWSGYFRYYGMDV | ND | ||||
| CLL246-H3 | EBV-B cells | 1-69 | 0.7 | GGTFSSYA | IIPILGIA | ARSDQNYNFWSGYFRYYGMDV | κ3-20 | 0.0 | QSVSSSY | GAS | QQYGSSPET | |
| CLL493 | B-CLL | PBMCs | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARDGTNLRGWIQLWPGAYFDY | κ1-33 | 0.0 | QDISNY | DAS | QQYDNLPYT |
| CLL493-1 | EBV-B cells | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARDGTNLRGWIQLWPGAYFDY | κ1-33 | 0.0 | QDISNY | DAS | QQYDNLPYT | |
| CLL526 | B-CLL | PBMCs | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARVGYYDFWSGYYPNYYYYGMDV | λ3-9 | 0.0 | NIGSKN | RDS | QVWDSSTV |
| CLL526-G10 | EBV-B cells | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARVGYYDFWSGYYPNYYYYGMDV | λ3-9 | 0.0 | NIGSKN | RDS | QVWDSSTV | |
| CLL815 | B-CLL | PBMCs | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARGYCSSTSCYLWAIGVWYFDL | ND | ||||
| CLL815-C6 | EBV-B cells | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARGYCSSTSCYLWAIGVWYFDL | κ3-11 | 0.0 | QSVSSY | DAS | QQRSNWPYT | |
| CLL861 | B-CLL | PBMCs | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARGGEYDYIWGSYRPNDAFDI | κ3-20 | 0.0 | QSVSSSY | GAS | QQYGSSPGT |
| CLL861-D2 | EBV-B cells | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARGGEYDYIWGSYRPNDAFDI | κ3-20 | 0.0 | QSVSSSY | GAS | QQYGSSPGT | |
| CLL1153 | B-CLL | PBMCs | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARDDSYYDFWSGWYY | κ3-15 | 0.0 | QSVSSN | GAS | QQYNNWPLYT |
| CLL1153-E5 | EBV-B cells | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARDDSYYDFWSGWYY | κ3-15 | 0.0 | QSVSSN | GAS | QQYNNWPLYT | |
| CLL1324 | B-CLL | PBMCs | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | AREASYGSGSYYQQYYYYYYMDV | λ3-19 | 0.0 | SLRSYY | GKN | NSRDSSGNPYV |
| CLL1324-C9 | EBV-B cells | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | AREASYGSGSYYQQYYYYYYMDV | λ3-19 | 0.0 | SLRSYY | GKN | NSRDSSGNPYV | |
| CLL1121 | B-CLL | PBMCs | 3-11 | 0.0 | GFTFSDYY | ISSSGSTI | ARDRRGYDFWSGYLGPTPQGDY | ND | ||||
| CLL1121-E11 | EBV-B cells | 3-11 | 0.0 | GFTFSDYY | ISSSGSTI | ARDRRGYDFWSGYLGPTPQGDY | κ2-30 | 0.0 | QSLVYSDGNTY | KVS | MQGTHWPPL | |
| CLL1193 | B-CLL | PBMCs | 3-13 | 6.3 | GFTFSNYD | IGTAGDT | VRGPRGYCSGDGCFSDSFDY | ND | ||||
| CLL1193-C9 | EBV-B cells | 3-13 | 6.3 | GFTFSNYD | IGTAGDT | VRGPRGYCSGDGCFSDSFDY | κ1-16 | 3.7 | QGINHH | AAS | QQYNNYPLT | |
| IGHV . | IGKV/IGLV* . | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CLL ID . | Cell line . | Source . | Family . | Mutation, % . | CDR1 . | CDR2 . | CDR3 . | Family . | Mutation, % . | CDR1 . | CDR2 . | CDR3 . |
| CLL246 | B-CLL | PBMCs | 1-69 | 0.3 | GGTFSSYA | IIPILGIA | ARSDQNYDFWSGYFRYYGMDV | ND | ||||
| CLL246-H3 | EBV-B cells | 1-69 | 0.7 | GGTFSSYA | IIPILGIA | ARSDQNYNFWSGYFRYYGMDV | κ3-20 | 0.0 | QSVSSSY | GAS | QQYGSSPET | |
| CLL493 | B-CLL | PBMCs | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARDGTNLRGWIQLWPGAYFDY | κ1-33 | 0.0 | QDISNY | DAS | QQYDNLPYT |
| CLL493-1 | EBV-B cells | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARDGTNLRGWIQLWPGAYFDY | κ1-33 | 0.0 | QDISNY | DAS | QQYDNLPYT | |
| CLL526 | B-CLL | PBMCs | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARVGYYDFWSGYYPNYYYYGMDV | λ3-9 | 0.0 | NIGSKN | RDS | QVWDSSTV |
| CLL526-G10 | EBV-B cells | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARVGYYDFWSGYYPNYYYYGMDV | λ3-9 | 0.0 | NIGSKN | RDS | QVWDSSTV | |
| CLL815 | B-CLL | PBMCs | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARGYCSSTSCYLWAIGVWYFDL | ND | ||||
| CLL815-C6 | EBV-B cells | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARGYCSSTSCYLWAIGVWYFDL | κ3-11 | 0.0 | QSVSSY | DAS | QQRSNWPYT | |
| CLL861 | B-CLL | PBMCs | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARGGEYDYIWGSYRPNDAFDI | κ3-20 | 0.0 | QSVSSSY | GAS | QQYGSSPGT |
| CLL861-D2 | EBV-B cells | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARGGEYDYIWGSYRPNDAFDI | κ3-20 | 0.0 | QSVSSSY | GAS | QQYGSSPGT | |
| CLL1153 | B-CLL | PBMCs | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARDDSYYDFWSGWYY | κ3-15 | 0.0 | QSVSSN | GAS | QQYNNWPLYT |
| CLL1153-E5 | EBV-B cells | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | ARDDSYYDFWSGWYY | κ3-15 | 0.0 | QSVSSN | GAS | QQYNNWPLYT | |
| CLL1324 | B-CLL | PBMCs | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | AREASYGSGSYYQQYYYYYYMDV | λ3-19 | 0.0 | SLRSYY | GKN | NSRDSSGNPYV |
| CLL1324-C9 | EBV-B cells | 1-69 | 0.0 | GGTFSSYA | IIPIFGTA | AREASYGSGSYYQQYYYYYYMDV | λ3-19 | 0.0 | SLRSYY | GKN | NSRDSSGNPYV | |
| CLL1121 | B-CLL | PBMCs | 3-11 | 0.0 | GFTFSDYY | ISSSGSTI | ARDRRGYDFWSGYLGPTPQGDY | ND | ||||
| CLL1121-E11 | EBV-B cells | 3-11 | 0.0 | GFTFSDYY | ISSSGSTI | ARDRRGYDFWSGYLGPTPQGDY | κ2-30 | 0.0 | QSLVYSDGNTY | KVS | MQGTHWPPL | |
| CLL1193 | B-CLL | PBMCs | 3-13 | 6.3 | GFTFSNYD | IGTAGDT | VRGPRGYCSGDGCFSDSFDY | ND | ||||
| CLL1193-C9 | EBV-B cells | 3-13 | 6.3 | GFTFSNYD | IGTAGDT | VRGPRGYCSGDGCFSDSFDY | κ1-16 | 3.7 | QGINHH | AAS | QQYNNYPLT | |
The B-CLL IGHV and IGKV/IGLV sequences were obtained from PBMCs as described previously.30 The IGHV and IGKV/IGLV sequences were obtained from sorted B cells of 9 cultured EBV-transformed B (EBV-B) cell lines. The IGHV and IGKV/IGLV mutations (%) were compared with germline according to IMGT.27
ND indicates not determined.
For clarity, only the dominant IGKV/IGLV sequences are shown for each cell line.