Table 1 Thermodynamic parameters of... | American Society of Hematology

Table 1

Thermodynamic parameters of VEGF/VEGFR-2 interactions determined by ITC*

	N	K_d, μM	ΔG, kcal/mol	ΔH, kcal/mol	−TΔS, kcal/mol	c†
VEGF-A₁₂₁/VEGFR-2 D23	2.01	0.093	−8.9	6.6	−15.5	281
VEGF-A₁₆₅/VEGFR-2 D23	1.89	0.17	−8.6	6.5	−15.1	150
VEGF-C/VEGFR-2 D23	1.91	0.20	−8.5	12.0	−20.5	131
VEGF-E/VEGFR-2 D23	2.21	0.28	−8.3	13.2	−21.5	91
VEGF-A₁₂₁/VEGFR-2 ECD	1.97	1.12	−7.5	10.4	−17.9	57
VEGF-A₁₆₅/VEGFR-2 ECD	2.06	2.67	−7.1	12.5	−19.6	24
VEGF-C/VEGFR-2 ECD	1.9	1.21	−7.5	14.8	−22.3	53
VEGF-E/VEGFR-2 ECD	2.09	5.99	−6.6	17.7	−24.3	11
			ΔΔG, kcal/mol	ΔΔH, kcal/mol	−TΔΔS, kcal/mol
VEGF-A₁₂₁‡			1.4	3.8	−2.4
VEGF-A₁₆₅‡			1.5	6.0	−4.5
VEGF-C‡			1.0	2.8	−1.8
VEGF-E‡			1.7	4.5	−2.8

	N	K_d, μM	ΔG, kcal/mol	ΔH, kcal/mol	−TΔS, kcal/mol	c†
VEGF-A₁₂₁/VEGFR-2 D23	2.01	0.093	−8.9	6.6	−15.5	281
VEGF-A₁₆₅/VEGFR-2 D23	1.89	0.17	−8.6	6.5	−15.1	150
VEGF-C/VEGFR-2 D23	1.91	0.20	−8.5	12.0	−20.5	131
VEGF-E/VEGFR-2 D23	2.21	0.28	−8.3	13.2	−21.5	91
VEGF-A₁₂₁/VEGFR-2 ECD	1.97	1.12	−7.5	10.4	−17.9	57
VEGF-A₁₆₅/VEGFR-2 ECD	2.06	2.67	−7.1	12.5	−19.6	24
VEGF-C/VEGFR-2 ECD	1.9	1.21	−7.5	14.8	−22.3	53
VEGF-E/VEGFR-2 ECD	2.09	5.99	−6.6	17.7	−24.3	11
			ΔΔG, kcal/mol	ΔΔH, kcal/mol	−TΔΔS, kcal/mol
VEGF-A₁₂₁‡			1.4	3.8	−2.4
VEGF-A₁₆₅‡			1.5	6.0	−4.5
VEGF-C‡			1.0	2.8	−1.8
VEGF-E‡			1.7	4.5	−2.8

N indicates the stoichiometry of ligand/receptor complexes.

Error estimation: N, ± 0.01; K_d, ± 5%; ΔG, ΔH, and −TΔS, ± 0.5 kcal mol⁻¹.

The c value is calculated as: c = N*[R2]/K_d.

ΔΔ values represent the difference between the ΔG/ΔH/−TΔS values between the full-length ECD and the D23 ligand complexes.