Bioassay of purified fVIII constructs with comparison to fVIII-deficient plasma
| FVIII construct . | Specific activity, U/mg* . | Intrinsic factor Xase assay† . | Endogenous thrombin potential‡ . | Peak thrombin generation‡ . |
|---|---|---|---|---|
| wt fVIII | 8075 | 100 | 6880 ± 760 | 780 ± 140 |
| R372A/R1689A fVIII | 36 | < 1 | 5330 ± 740 | 260 ± 37 |
| V634M fVIII | 57 | < 1 | 5640 ± 610 | 420 ± 87 |
| fVIII-deficient plasma | 5120 ± 1220 | 280 ± 95 |
| FVIII construct . | Specific activity, U/mg* . | Intrinsic factor Xase assay† . | Endogenous thrombin potential‡ . | Peak thrombin generation‡ . |
|---|---|---|---|---|
| wt fVIII | 8075 | 100 | 6880 ± 760 | 780 ± 140 |
| R372A/R1689A fVIII | 36 | < 1 | 5330 ± 740 | 260 ± 37 |
| V634M fVIII | 57 | < 1 | 5640 ± 610 | 420 ± 87 |
| fVIII-deficient plasma | 5120 ± 1220 | 280 ± 95 |
FVIII activity was determined by 1-stage coagulation assay as described in “FVIII bioassays.”
FVIII-dependent intrinsic factor Xase activity was measured as described in “FVIII bioassays” and expressed relative to wt fVIII.
Thrombin generation assays were performed as described in “FVIII bioassays.” Errors represent sample SDs derived from triplicates from each of 2 independent experiments.