SNAREs in human and murine platelets
| SNARE . | Molecules/platelet . | |
|---|---|---|
| Human . | Murine . | |
| t-SNAREs | ||
| Syntaxin-2 | 1579 ± 429 | 1766 ± 121 |
| Syntaxin-4 | 9633 ± 75 | 1625 ± 184 |
| Syntaxin-7 | 3861 ± 564 | 3707 ± 372 |
| Syntaxin-11 | 51 717 ± 5459 | 46 966 ± 5516 |
| SNAP-23 | 19 374 ± 5384 | 5363 ± 798 |
| v-SNAREs | ||
| VAMP-2* | < 806 | 7629 ± 89 |
| VAMP-3* | 4588 ± 658 | 2197 ± 120 |
| VAMP-7* | 3766 ± 696 | 2712 ± 230 |
| VAMP-8* | 6590 ± 370 | 8360 ± 110 |
| SNARE . | Molecules/platelet . | |
|---|---|---|
| Human . | Murine . | |
| t-SNAREs | ||
| Syntaxin-2 | 1579 ± 429 | 1766 ± 121 |
| Syntaxin-4 | 9633 ± 75 | 1625 ± 184 |
| Syntaxin-7 | 3861 ± 564 | 3707 ± 372 |
| Syntaxin-11 | 51 717 ± 5459 | 46 966 ± 5516 |
| SNAP-23 | 19 374 ± 5384 | 5363 ± 798 |
| v-SNAREs | ||
| VAMP-2* | < 806 | 7629 ± 89 |
| VAMP-3* | 4588 ± 658 | 2197 ± 120 |
| VAMP-7* | 3766 ± 696 | 2712 ± 230 |
| VAMP-8* | 6590 ± 370 | 8360 ± 110 |
The number of molecules of the indicated SNAREs per platelet was determined by quantitative Western blotting using recombinant proteins to generate a standard curve. All measurements were made at least 3 times. The mass, in nanograms per 5 × 107 platelets, was determined based on comparison with the standard curves of recombinant proteins. This data were used to calculate the number of molecules per platelet using the molecular weight for SNAP-23 (23 kDa), Syntaxin-2 (33 kDa), Syntaxin-4 (34 kDa), Syntaxin-7 (30 kDa), and Syntaxin-11 (33 kDa).
Number of VAMP molecules per platelet from Graham et al.7