Motif-specific phosphorylation by active recombinant ERK2
| Protein description . | Modified peptide sequence . | Phospho- residue . | AA + ERK2 vs AA . | P . | SS + U012 6 vs SS . | P . |
|---|---|---|---|---|---|---|
| α-adducin | 355-[pS]PG[pS]PVGEGTGSPPK-369 | S355, S358 | −1.71 | .035 | 1.47 | .122 |
| α-adducin | 661-EEEAHRPP[pS]PTEAPTEASP | S669, S707 | −1.69 | .025 | 1.60 | .074 |
| EPAPDPAPVAEEAAPSAVEEGAA | ||||||
| ADPG[pS]DGSPGK-713 | ||||||
| β-adducin | 584-ETAPEEPG[pS]PAK[pS]AP | S592, S596, | −2.36 | 4.21 × 10−06 | 1.91 | 3.87 × 10−09 |
| A[pS]PVQSPAK-607 | S600 | |||||
| β-adducin | 584-ETAPEEPGSPAK[pS]APA[pS]PV | S596, S600 | 1.75 | .020 | 1.82 | .018 |
| QSPAK-607 | ||||||
| β-adducin | 584-ETAPEEPG[pS]PAK[pS]APA | S592, S596 | 1.68 | .037 | 1.68 | .010 |
| SPVQSPAK-607 | ||||||
| Dematin | 90-[pS]TSPPP[pS]PEVWADSR-104 | S90, S96 | −1.76 | 9.46 × 10−09 | 1.48 | 4.98 × 10−04 |
| Protein description . | Modified peptide sequence . | Phospho- residue . | AA + ERK2 vs AA . | P . | SS + U012 6 vs SS . | P . |
|---|---|---|---|---|---|---|
| α-adducin | 355-[pS]PG[pS]PVGEGTGSPPK-369 | S355, S358 | −1.71 | .035 | 1.47 | .122 |
| α-adducin | 661-EEEAHRPP[pS]PTEAPTEASP | S669, S707 | −1.69 | .025 | 1.60 | .074 |
| EPAPDPAPVAEEAAPSAVEEGAA | ||||||
| ADPG[pS]DGSPGK-713 | ||||||
| β-adducin | 584-ETAPEEPG[pS]PAK[pS]AP | S592, S596, | −2.36 | 4.21 × 10−06 | 1.91 | 3.87 × 10−09 |
| A[pS]PVQSPAK-607 | S600 | |||||
| β-adducin | 584-ETAPEEPGSPAK[pS]APA[pS]PV | S596, S600 | 1.75 | .020 | 1.82 | .018 |
| QSPAK-607 | ||||||
| β-adducin | 584-ETAPEEPG[pS]PAK[pS]APA | S592, S596 | 1.68 | .037 | 1.68 | .010 |
| SPVQSPAK-607 | ||||||
| Dematin | 90-[pS]TSPPP[pS]PEVWADSR-104 | S90, S96 | −1.76 | 9.46 × 10−09 | 1.48 | 4.98 × 10−04 |
Fold changes in phosphorylation for peptides containing the ERK consensus motif [PV]x[pST]P were presented. Phopshorylation is up-regulated in healthy RBCs (AA) with addition of active ERK2 and down-regulated in SS RBCs (SS) with addition of the MEK inhibitor U0126.