Activating and inhibitory receptor repertoire on splenic and developing BM NK cells
| . | Eri1 . | II . | III . | IV . | V . | Spleen . |
|---|---|---|---|---|---|---|
| Ly49H | +/+ | 1.3 (0.1) | 11.0 (2.5) | 59.6 (3.4) | 76.7 (3.8) | 71.0 (3.5) |
| −/− | 0.5 (0.3) | 4.6 (2.0) | 40.8 (0.6) | 61.4 (1.7) | 50.1 (3.5) | |
| Ly49D | +/+ | 2.8 (0.5) | 13.6 (2.0) | 54.6 (2.9) | 55.4 (1.5) | 59.2 (1.0) |
| −/− | 2.3 (0.7) | 6.9 (1.8) | 47.7 (1.8) | 53.1 (1.6) | 48.4 (1.4) | |
| Ly49G2 | +/+ | 4.2 (1.6) | 15.9 (3.1) | 50.4 (5.6) | 49.2 (4.9) | 37.3 (2.7) |
| −/− | 4.2 (1.6) | 10.2 (2.4) | 56.6 (2.3) | 56.0 (5.2) | 38.1 (6.0) | |
| Ly49C/I | +/+ | 5.5 (0.7) | 19.0 (4.9) | 30.9 (1.2) | 34.6 (1.1) | 28.9 (3.5) |
| −/− | 3.5 (1.2) | 7.4 (1.2) | 26.0 (1.7) | 30.9 (1.9) | 24.4 (4.0) | |
| NKG2A/C/E | +/+ | 59.8 (0.7) | 60.1 (2.0) | 44.9 (0.7) | 45.5 (1.1) | 44.0 (1.3) |
| −/− | 60.7 (3.2) | 60.0 (1.8) | 55.9 (4.2) | 51.8 (2.8) | 52.2 (0.9) |
| . | Eri1 . | II . | III . | IV . | V . | Spleen . |
|---|---|---|---|---|---|---|
| Ly49H | +/+ | 1.3 (0.1) | 11.0 (2.5) | 59.6 (3.4) | 76.7 (3.8) | 71.0 (3.5) |
| −/− | 0.5 (0.3) | 4.6 (2.0) | 40.8 (0.6) | 61.4 (1.7) | 50.1 (3.5) | |
| Ly49D | +/+ | 2.8 (0.5) | 13.6 (2.0) | 54.6 (2.9) | 55.4 (1.5) | 59.2 (1.0) |
| −/− | 2.3 (0.7) | 6.9 (1.8) | 47.7 (1.8) | 53.1 (1.6) | 48.4 (1.4) | |
| Ly49G2 | +/+ | 4.2 (1.6) | 15.9 (3.1) | 50.4 (5.6) | 49.2 (4.9) | 37.3 (2.7) |
| −/− | 4.2 (1.6) | 10.2 (2.4) | 56.6 (2.3) | 56.0 (5.2) | 38.1 (6.0) | |
| Ly49C/I | +/+ | 5.5 (0.7) | 19.0 (4.9) | 30.9 (1.2) | 34.6 (1.1) | 28.9 (3.5) |
| −/− | 3.5 (1.2) | 7.4 (1.2) | 26.0 (1.7) | 30.9 (1.9) | 24.4 (4.0) | |
| NKG2A/C/E | +/+ | 59.8 (0.7) | 60.1 (2.0) | 44.9 (0.7) | 45.5 (1.1) | 44.0 (1.3) |
| −/− | 60.7 (3.2) | 60.0 (1.8) | 55.9 (4.2) | 51.8 (2.8) | 52.2 (0.9) |
WT CD45.1+ lethally irradiated hosts were reconstituted with a 1:1 mixture of fetal liver cells from congenic WT (CD45.1+CD45.2+) and Eri1−/− (CD45.2+) mice and analyzed 12 weeks later. Frequencies of NK-cell receptor expression on freshly isolated WT and Eri1−/− splenic NK cells (NK1.1+CD3ϵ−) and stage II-V developing BM NK cells (gating strategy shown in supplemental Figure 2A) are shown. Mean (SD) for 3 mice are listed.