Critical values of CD69 and CD154 suppression by freshly isolated Tregs as a diagnostic test for inhibition of proliferation at 96 hours
| Marker and values . | Sensitivity, % (95% CI) . | Specificity, % (95% CI) . | PPV, % . | NPV, % . | LR+ . | LR− . |
|---|---|---|---|---|---|---|
| CD69 | ||||||
| Lower quartile: > 9.9% | 93 (68-100) | 40 (20-64) | 61 | 86 | 1.6 | 0.2 |
| Median: > 28.6% | 67 (42-85) | 67 (42-85) | 67 | 67 | 2.0 | 0.5 |
| Upper quartile: > 39.6% | 40 (20-64) | 93 (68-100) | 86 | 61 | 6.0 | 0.6 |
| Minimum cost: 37.4% | 53 (30-75) | 93 (68-100) | 89 | 67 | 8.0 | 0.5 |
| CD154 | ||||||
| Lower quartile: > 17.4% | 86 (61-97) | 40 (20-64) | 59 | 75 | 1.4 | 0.3 |
| Median: > 37.2% | 67 (42-85) | 73 (48-89) | 71 | 69 | 2.5 | 0.5 |
| Upper quartile: > 48.4% | 33 (15-59) | 93 (68-100) | 83 | 58 | 5.0 | 0.7 |
| Minimum cost: 43% | 53 (30-75) | 93 (68-100) | 89 | 67 | 8.0 | 0.5 |
| Marker and values . | Sensitivity, % (95% CI) . | Specificity, % (95% CI) . | PPV, % . | NPV, % . | LR+ . | LR− . |
|---|---|---|---|---|---|---|
| CD69 | ||||||
| Lower quartile: > 9.9% | 93 (68-100) | 40 (20-64) | 61 | 86 | 1.6 | 0.2 |
| Median: > 28.6% | 67 (42-85) | 67 (42-85) | 67 | 67 | 2.0 | 0.5 |
| Upper quartile: > 39.6% | 40 (20-64) | 93 (68-100) | 86 | 61 | 6.0 | 0.6 |
| Minimum cost: 37.4% | 53 (30-75) | 93 (68-100) | 89 | 67 | 8.0 | 0.5 |
| CD154 | ||||||
| Lower quartile: > 17.4% | 86 (61-97) | 40 (20-64) | 59 | 75 | 1.4 | 0.3 |
| Median: > 37.2% | 67 (42-85) | 73 (48-89) | 71 | 69 | 2.5 | 0.5 |
| Upper quartile: > 48.4% | 33 (15-59) | 93 (68-100) | 83 | 58 | 5.0 | 0.7 |
| Minimum cost: 43% | 53 (30-75) | 93 (68-100) | 89 | 67 | 8.0 | 0.5 |
PPV indicates positive predictive value; NPV, negative predictive value; LR+, likelihood ratio of a positive result; and LR−, likelihood ratio of a negative result.