Dissociation rate values for the tether bond between GPIbα receptor and wild type or mutated D′A3
| Shear rate, s−1 . | koff . | |||
|---|---|---|---|---|
| 400 . | 600 . | 1000 . | 2000 . | |
| wtD′A3 | 6.7 ± 1.3 | 7.1 ± 1.1 | 7.1 ± 1.4 | 9.2 ± 1.6 |
| DC-D′A3 | 5.4 ± 0.9 | 4.7 ± 0.6 | 5.7 ± 0.9 | 3.7 ± 0.4 |
| T1255A-D′A3 | 6.6 ± .0.1 | 6.6 ± 0.8 | 6 ± 0.2 | 7.4 ± 1.7 |
| Clus1-D′A3 | 5.5 ± 1.0 | 6.4 ± 1.2 | 5.1 ± 0.5 | 7.5 ± 1.6 |
| T1468A | 5.9 ± 0.9 | 6.9 ± 0.8 | 6.9 ± 0.9 | 7.9 ± 1.5 |
| Shear rate, s−1 . | koff . | |||
|---|---|---|---|---|
| 400 . | 600 . | 1000 . | 2000 . | |
| wtD′A3 | 6.7 ± 1.3 | 7.1 ± 1.1 | 7.1 ± 1.4 | 9.2 ± 1.6 |
| DC-D′A3 | 5.4 ± 0.9 | 4.7 ± 0.6 | 5.7 ± 0.9 | 3.7 ± 0.4 |
| T1255A-D′A3 | 6.6 ± .0.1 | 6.6 ± 0.8 | 6 ± 0.2 | 7.4 ± 1.7 |
| Clus1-D′A3 | 5.5 ± 1.0 | 6.4 ± 1.2 | 5.1 ± 0.5 | 7.5 ± 1.6 |
| T1468A | 5.9 ± 0.9 | 6.9 ± 0.8 | 6.9 ± 0.9 | 7.9 ± 1.5 |
koff values were calculated based on the duration of transient tethers at shear rates between 400 and 2000 seconds−1. Values are mean ± SEM of 3 separate experiments. Results were analyzed by ANOVA followed by Bonferroni adjustment for multiple comparisons.