Levels of platelet glycoproteins in wild-type and RhoA−/− mice
| . | Wild-type . | RhoA−/− . | P . |
|---|---|---|---|
| Mean FSC* | 326 ± 11 | 486 ± 32 | < .001 |
| GPIb | 353 ± 22 | 349 ± 14 | NS |
| GPV | 302 ± 8 | 329 ± 25 | NS |
| GPIX | 503 ± 16 | 530 ± 31 | NS |
| CD9 | 1433 ± 42 | 1441 ± 36 | NS |
| GPVI | 48 ± 5 | 56 ± 5 | NS |
| α2 | 61 ± 3 | 62 ± 1 | NS |
| β1 | 163 ± 4 | 183 ± 3 | < .001 |
| αIIbβ3 | 457 ± 10 | 590 ± 39 | < .01 |
| CLEC-2 | 155 ± 5 | 180 ± 12 | < .05 |
| . | Wild-type . | RhoA−/− . | P . |
|---|---|---|---|
| Mean FSC* | 326 ± 11 | 486 ± 32 | < .001 |
| GPIb | 353 ± 22 | 349 ± 14 | NS |
| GPV | 302 ± 8 | 329 ± 25 | NS |
| GPIX | 503 ± 16 | 530 ± 31 | NS |
| CD9 | 1433 ± 42 | 1441 ± 36 | NS |
| GPVI | 48 ± 5 | 56 ± 5 | NS |
| α2 | 61 ± 3 | 62 ± 1 | NS |
| β1 | 163 ± 4 | 183 ± 3 | < .001 |
| αIIbβ3 | 457 ± 10 | 590 ± 39 | < .01 |
| CLEC-2 | 155 ± 5 | 180 ± 12 | < .05 |
Expression of glycoproteins on the platelet surface was determined by flow cytometry. Diluted whole blood from the indicated mice was incubated with FITC-labeled antibodies at saturating concentrations for 15 minutes at room temperature, and platelets were analyzed directly. Data are expressed as mean fluorescence intensity ± SD (n = 4) and are representative of 3 individual experiments.
Mean platelet size (mean FSC) was determined by forward-scatter characteristics.