Targeted nucleases
| Nuclease . | DNA-binding domain . | Active configuration . | Cleaving enzyme; break characteristic . | Target range . |
|---|---|---|---|---|
| Homing endonucleases (meganucleases) | 5 families: LAGLIDADG, GIY-YIG, HNH, His-Cys box, and PD- (D/E)XK | Homodimeric or monomeric | 3′ overhang | 14-40 bases |
| ZFN | Zinc-finger motif | Protein dimer | FokI; 5′ overhang | 9-18 bases per monomer |
| TALEN | Repeat variable diresidue | Protein dimer | FokI; 5′ overhang | 12-31 bases per monomer |
| CRISPR/Cas9* | CRISPR-RNA and trans-activating RNA | RNA/protein complex | Cas9; Blunt | 17-20 bases |
| Nuclease . | DNA-binding domain . | Active configuration . | Cleaving enzyme; break characteristic . | Target range . |
|---|---|---|---|---|
| Homing endonucleases (meganucleases) | 5 families: LAGLIDADG, GIY-YIG, HNH, His-Cys box, and PD- (D/E)XK | Homodimeric or monomeric | 3′ overhang | 14-40 bases |
| ZFN | Zinc-finger motif | Protein dimer | FokI; 5′ overhang | 9-18 bases per monomer |
| TALEN | Repeat variable diresidue | Protein dimer | FokI; 5′ overhang | 12-31 bases per monomer |
| CRISPR/Cas9* | CRISPR-RNA and trans-activating RNA | RNA/protein complex | Cas9; Blunt | 17-20 bases |
Characteristics listed here describe Streptococcus pyogenes Cas9. Other Cas9 variants and orthologs have related but not identical properties.