CD107a and intracellular cytokine staining of PBMCs and BM cells from PIN 5, 7, 12, 15, and 25
| PIN . | Tumor-antigen peptide . | % Expression of CD8+ T cells, minus the % in the unstimulated . | |||
|---|---|---|---|---|---|
| CD107α . | IL-2 . | TNFα . | IFNγ . | ||
| 5 (PBMCs) | MAGE-A2157-166 | 0.1 | 0.012 | 0.014 | 0.034 |
| 5 (PBMCs) | MAGE-A3112-120 | 0 | 0 | 0 | 0.042 |
| 12 (PBMCs) | MAGE-A1161-169 | 0.03 | 0.03 | 0 | 0 |
| 12 (PBMCs) | MAGE-A3168-176/MAGE-A2212-220 | 0.1 | 0 | 0.014 | 0 |
| 7 (PBMCs) | BAGE-12-10 | 0.013 | 0 | 0 | 0.03 |
| 7 (BM) | BAGE-12-10 | 0.01 | 0 | 0 | 0.05 |
| 15 (PBMCs) | MAGE-A3168-176/MAGE-A196-104 | ND | 0 | 0.02 | 0.09 |
| 15 (BM) | MAGE-A3168-176 | 0.044 | 0.09 | 0.5 | 0.251 |
| 15 (BM) | MAGE-A196-104 | 0.35 | 0.035 | 0.5 | 0.08 |
| 25 (BM) | MAGE-A397-105 | 0.06 | 0 | 0.8 | 0 |
| 25 (BM) | WT-1235-243 | 0.3 | 0.06 | 0.9 | 0 |
| PIN . | Tumor-antigen peptide . | % Expression of CD8+ T cells, minus the % in the unstimulated . | |||
|---|---|---|---|---|---|
| CD107α . | IL-2 . | TNFα . | IFNγ . | ||
| 5 (PBMCs) | MAGE-A2157-166 | 0.1 | 0.012 | 0.014 | 0.034 |
| 5 (PBMCs) | MAGE-A3112-120 | 0 | 0 | 0 | 0.042 |
| 12 (PBMCs) | MAGE-A1161-169 | 0.03 | 0.03 | 0 | 0 |
| 12 (PBMCs) | MAGE-A3168-176/MAGE-A2212-220 | 0.1 | 0 | 0.014 | 0 |
| 7 (PBMCs) | BAGE-12-10 | 0.013 | 0 | 0 | 0.03 |
| 7 (BM) | BAGE-12-10 | 0.01 | 0 | 0 | 0.05 |
| 15 (PBMCs) | MAGE-A3168-176/MAGE-A196-104 | ND | 0 | 0.02 | 0.09 |
| 15 (BM) | MAGE-A3168-176 | 0.044 | 0.09 | 0.5 | 0.251 |
| 15 (BM) | MAGE-A196-104 | 0.35 | 0.035 | 0.5 | 0.08 |
| 25 (BM) | MAGE-A397-105 | 0.06 | 0 | 0.8 | 0 |
| 25 (BM) | WT-1235-243 | 0.3 | 0.06 | 0.9 | 0 |
BAGE indicates B melanoma Ag; MAGE, melanoma-associated Ag; PIN, personal identification number; and WT-1, Wilm tumor Ag 1.