Table 1

ER71 is cell-autonomously required for blood and endothelial cell generation

TailPBBMSpleenHeartKidneyLiverLung Vecad+
Mouse no. %129 %129 %129 %129 %129 %129 %129 %129 
    6155 25 0* 0* 0* 28 37 41 0* 
    6160 28 0* 0* 0* 22 39 21 0* 
    6154 36 0* 0* 0* 36 38 43 0* 
    6175 27 61 65 59 44 48 39 32 
    6170 35 63 72 63 50 51 47 38 
    6168 40 54 55 53 56 44 59 46 
TailPBBMSpleenHeartKidneyLiverLung Vecad+
Mouse no. %129 %129 %129 %129 %129 %129 %129 %129 
    6155 25 0* 0* 0* 28 37 41 0* 
    6160 28 0* 0* 0* 22 39 21 0* 
    6154 36 0* 0* 0* 36 38 43 0* 
    6175 27 61 65 59 44 48 39 32 
    6170 35 63 72 63 50 51 47 38 
    6168 40 54 55 53 56 44 59 46 

Different tissues from chimeric mice generated with Er71+/+ or Er71−/− ES cells were analyzed for the ES contribution by utilizing 129 strain specific micro-satellite sequence. Numbers indicate the percentage of 129 strain DNA sequence, which represents ES derived cells. Numbers 6155, 6160 and 6154 are chimeric mice generated with Er71-/- ES cells and 6175, 6170 and 6168 are chimeric mice generated with Er71+/+ ES cells.

0* indicates < 5% detection limit of 129 contribution by calculating the 129/BL6 peak height ratio with the standard curve, which was produced by mixing 129 and BL6 DNA. Tissues analyzed include peripheral blood (PB), BM, and VE-cadherin+ cells (lung Vecad+) sorted from lung tissue.

View Large
Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal