Description of posttranslational modifications in UM-CLL cells after anti-IgM stimulation in proteins with multiple isoforms identified
| Gene name . | Protein name . | No. of isoforms identified . | Significant statistical variation in US samples vs M-CLL cells . | Isoform variation profile after stimulation in UM-CLL cells . | Significant statistical variation after stimulation in UM-CLL cells . | Biologic hypothesis in UM-CLL cells . |
|---|---|---|---|---|---|---|
| LMNB1 | Lamin-B1 | 6 | — | 4 increased (acid) 2 decreased (basic) | More acidic isoform increased | Constitutive phosphorylation enhanced after anti-IgM stimulation |
| LMNB2 | Lamin-B2 | 2 | — | 2 decreased | 1 basic isoform decreased | Decreased LMNB2 amount after anti-IgM stimulation |
| LCP1 | Plastin-2 | 3 | — | 1 increased (acid) 2 decreased (basic) | More acidic isoform increased | Phosphorylation after anti-IgM stimulation |
| VIM | Vimentin | 5 | — | 3 increased (acid) 2 decreased (basic) | More acidic isoform increased | Phosphorylation after anti-IgM stimulation |
| HCLS1 | Hematopoietic lineage cell-specific protein | 4 | More basic isoform decreased | 1 increased (acid) 1 decreased (basic) 2 no modified | More acidic isoform increased | Constitutive phosphorylation enhanced after anti-IgM stimulation |
| LSP1 | Lymphocyte-specific protein 1 | 6 | — | 2 increased (acid) 4 decreased (basic) | More acidic isoform increased | Phosphorylation after anti-IgM stimulation |
| DYNC1I2 | Cytoplasmic dynein 1 intermediate chain 2 | 3 | — | 2 isoforms (acid) increased 1 isoform (basic) decreased | More acidic isoform increased | Phosphorylation after anti-IgM stimulation |
| HNRNPK | Heterogeneous nuclear ribonucleoprotein K | 9 | 1 isoform increased 1 isoform decreased | 5 increased 4 decreased | 1 isoform increased | Phosphorylation after anti-IgM stimulation |
| HNRNPC | Heterogeneous nuclear ribonucleoproteins C1/C2 | 4 | — | 1 isoform decreased 3 no modified | 1 isoform decreased | — |
| ESD | S-formylglutathione hydrolase | 2 | 2 isoforms increased | — | — | Constitutive increase of ESD amount |
| TALDO1 | Transaldolase | 4 | — | 3 decreased 1 no modified | 1 isoform decreased | Decreased amount of transaldolase after anti-IgM stimulation |
| HSPD1 | 60-kDa heat shock protein; mitochondrial | 4 | 1 isoform increased | 1 isoform increased 2 no modified 1 decreased | — | — |
| PDCD4 | Programmed cell death protein 4 | 3 | 1 isoform decreased | 3 isoforms decreased | 1 isoform decreased | Decreased PDCD4 amount at baseline and more after anti-IgM activation |
| PSME1 | Proteasome activator complex subunit 1 | 3 | — | 2 isoforms increased 1 no modified | 1 isoform increased | Increased amount of PSME1 after anti-IgM stimulation |
| Gene name . | Protein name . | No. of isoforms identified . | Significant statistical variation in US samples vs M-CLL cells . | Isoform variation profile after stimulation in UM-CLL cells . | Significant statistical variation after stimulation in UM-CLL cells . | Biologic hypothesis in UM-CLL cells . |
|---|---|---|---|---|---|---|
| LMNB1 | Lamin-B1 | 6 | — | 4 increased (acid) 2 decreased (basic) | More acidic isoform increased | Constitutive phosphorylation enhanced after anti-IgM stimulation |
| LMNB2 | Lamin-B2 | 2 | — | 2 decreased | 1 basic isoform decreased | Decreased LMNB2 amount after anti-IgM stimulation |
| LCP1 | Plastin-2 | 3 | — | 1 increased (acid) 2 decreased (basic) | More acidic isoform increased | Phosphorylation after anti-IgM stimulation |
| VIM | Vimentin | 5 | — | 3 increased (acid) 2 decreased (basic) | More acidic isoform increased | Phosphorylation after anti-IgM stimulation |
| HCLS1 | Hematopoietic lineage cell-specific protein | 4 | More basic isoform decreased | 1 increased (acid) 1 decreased (basic) 2 no modified | More acidic isoform increased | Constitutive phosphorylation enhanced after anti-IgM stimulation |
| LSP1 | Lymphocyte-specific protein 1 | 6 | — | 2 increased (acid) 4 decreased (basic) | More acidic isoform increased | Phosphorylation after anti-IgM stimulation |
| DYNC1I2 | Cytoplasmic dynein 1 intermediate chain 2 | 3 | — | 2 isoforms (acid) increased 1 isoform (basic) decreased | More acidic isoform increased | Phosphorylation after anti-IgM stimulation |
| HNRNPK | Heterogeneous nuclear ribonucleoprotein K | 9 | 1 isoform increased 1 isoform decreased | 5 increased 4 decreased | 1 isoform increased | Phosphorylation after anti-IgM stimulation |
| HNRNPC | Heterogeneous nuclear ribonucleoproteins C1/C2 | 4 | — | 1 isoform decreased 3 no modified | 1 isoform decreased | — |
| ESD | S-formylglutathione hydrolase | 2 | 2 isoforms increased | — | — | Constitutive increase of ESD amount |
| TALDO1 | Transaldolase | 4 | — | 3 decreased 1 no modified | 1 isoform decreased | Decreased amount of transaldolase after anti-IgM stimulation |
| HSPD1 | 60-kDa heat shock protein; mitochondrial | 4 | 1 isoform increased | 1 isoform increased 2 no modified 1 decreased | — | — |
| PDCD4 | Programmed cell death protein 4 | 3 | 1 isoform decreased | 3 isoforms decreased | 1 isoform decreased | Decreased PDCD4 amount at baseline and more after anti-IgM activation |
| PSME1 | Proteasome activator complex subunit 1 | 3 | — | 2 isoforms increased 1 no modified | 1 isoform increased | Increased amount of PSME1 after anti-IgM stimulation |
— indicates not applicable.