Most differentially expressed peptides between US and S M-CLL cells
| Spot no.* . | Fold change† . | Accession no.‡ . | Protein name§ . | Gene‖ . | t test¶ . | q value¶ . | Mascot score# . | Sequence cover, %# . | No. of peptides matched# . | Theoretical (pI/MW**) . | Observed (pI/MW††) . | Function‡‡ . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1903 | 1.6§§ | P30084 | Enoyl CoA hydratase, mitochondrial | ECHS1 | .02 | < .01 | 441 | 36 | 9 | 8.34/31 823 | 5.84/24 268 | Catalytic activity, metabolism, energy pathways |
| 1926 | 1.6§§ | O75489 | NADH dehydrogenase (ubiquinone) iron-sulfur protein 3 | NDUFS3 | .02 | < .01 | 143 | 45 | 12 | 6.99/30 337 | 5.68/23 542 | Catalytic activity, metabolism, energy pathways |
| 1873 | 1.6§§ | P43487 | Ran-specific GTPase-activating protein | RANBP1 | .04 | < .01 | 177 | 14 | 5 | 5.19/23 179 | 5.28/24 843 | Cell communication, signal transduction |
| 1861 | 1.9§§ | Q06323 | Proteasome activator complex subunit 1 | PSME1 | .02 | < .01 | 125 | 50 | 12 | 5.78/28 876 | 5.68/25 194 | Ubiquitin-specific protease activity, protein metabolism |
| 1933 | 2.7§§ | Q9GZP4 | UPF0424 protein C1orf128 | C1orf128 | .02 | < .01 | 101 | 23 | 3 | 5.47/24 391 | 5.65/23 432 | Unknown |
| 1851 | 2.7§§ | P21964 | Catechol O-methyltransferase | COMT | .02 | < .01 | 186 | 19 | 3 | 5.26/30 037 | 5.34/25 550 | Metabolism, energy pathways |
| Spot no.* . | Fold change† . | Accession no.‡ . | Protein name§ . | Gene‖ . | t test¶ . | q value¶ . | Mascot score# . | Sequence cover, %# . | No. of peptides matched# . | Theoretical (pI/MW**) . | Observed (pI/MW††) . | Function‡‡ . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1903 | 1.6§§ | P30084 | Enoyl CoA hydratase, mitochondrial | ECHS1 | .02 | < .01 | 441 | 36 | 9 | 8.34/31 823 | 5.84/24 268 | Catalytic activity, metabolism, energy pathways |
| 1926 | 1.6§§ | O75489 | NADH dehydrogenase (ubiquinone) iron-sulfur protein 3 | NDUFS3 | .02 | < .01 | 143 | 45 | 12 | 6.99/30 337 | 5.68/23 542 | Catalytic activity, metabolism, energy pathways |
| 1873 | 1.6§§ | P43487 | Ran-specific GTPase-activating protein | RANBP1 | .04 | < .01 | 177 | 14 | 5 | 5.19/23 179 | 5.28/24 843 | Cell communication, signal transduction |
| 1861 | 1.9§§ | Q06323 | Proteasome activator complex subunit 1 | PSME1 | .02 | < .01 | 125 | 50 | 12 | 5.78/28 876 | 5.68/25 194 | Ubiquitin-specific protease activity, protein metabolism |
| 1933 | 2.7§§ | Q9GZP4 | UPF0424 protein C1orf128 | C1orf128 | .02 | < .01 | 101 | 23 | 3 | 5.47/24 391 | 5.65/23 432 | Unknown |
| 1851 | 2.7§§ | P21964 | Catechol O-methyltransferase | COMT | .02 | < .01 | 186 | 19 | 3 | 5.26/30 037 | 5.34/25 550 | Metabolism, energy pathways |
Spot numbers refer to Master gel spots.
FC was calculated using DeCyder Version 6.5 software: negative FC indicates that the polypeptide spot is underexpressed in S samples compared with US; and positive FC, the spot is overexpressed in S M-CLL cells.
Accession number in UniProt database (www.uniprot.org).
Full protein name recommended by UniProt consortium.
Gene names according to HUGO gene nomenclature.
P value from t test was calculated using DeCyder Version 6.5 software; q-value was calculated using SAM.
Mascot score indicates the confidence of protein identification using the Mascot search engine (www.matrixscience.com), depending on amino acid sequence coverage (in %) and on the number of matched peptides used for identification in the Swiss-Prot database (www.expasy.org/sprot).
Theoretic isoelectric point (pI) and molecular weight obtained from the Mascot database.
Observed molecular weight and pI calculated by DeCyder Version 6.5 software, according to location in the gel.
Protein function and biologic process were assigned in accordance with the Human Protein Reference Database (www.hprd.org).
Protein isoforms up-regulated in S M-CLL cells.