Disease subtypes, molecular/cytogenetic data, and CalcuSyn synergy of ex vivo–cultured patient samples
| Patient no. . | FAB subtype/CML phase . | Cytogenetics/mutation . | Blast, % . | CI at 380nM MK . | AraC for maximum synergy (CI) at 380nM MK, nM . |
|---|---|---|---|---|---|
| AML 1 | M6 | * | * | ||
| AML 2 | M4 | 78 | * | * | |
| AML 4 | M5 | * | * | ||
| AML 6 | M2 | 74 | * | * | |
| AML 7 | Inv3, 5q−, −7 | 60-70 | 0.41 | 80 | |
| AML 8 | AMML | Diploid | 60 | 0.22 | 14 |
| AML 9 | M5 | 11q23 | 0.43 | 120 | |
| MDS 10 | Evolving to AML | Diploid | 10-15 | 0.24 | 9 |
| MDS 11 | Diploid | 5 | 0.45† | 80 | |
| CML 12 | CP (> 70% Ph+) | t(9;22) | 1 | 0.42 | 1100 |
| CML 13 | AP | t(9;22) | 5 | 0.21 | 40 |
| CML 14 | BC, refractory | 7q−, inv3 | 21 | * | * |
| CML 3 | BC | t(9;22), (T315I) | * | * | |
| CML 5 | AP | t(9;22), (F359V) | * | * |
| Patient no. . | FAB subtype/CML phase . | Cytogenetics/mutation . | Blast, % . | CI at 380nM MK . | AraC for maximum synergy (CI) at 380nM MK, nM . |
|---|---|---|---|---|---|
| AML 1 | M6 | * | * | ||
| AML 2 | M4 | 78 | * | * | |
| AML 4 | M5 | * | * | ||
| AML 6 | M2 | 74 | * | * | |
| AML 7 | Inv3, 5q−, −7 | 60-70 | 0.41 | 80 | |
| AML 8 | AMML | Diploid | 60 | 0.22 | 14 |
| AML 9 | M5 | 11q23 | 0.43 | 120 | |
| MDS 10 | Evolving to AML | Diploid | 10-15 | 0.24 | 9 |
| MDS 11 | Diploid | 5 | 0.45† | 80 | |
| CML 12 | CP (> 70% Ph+) | t(9;22) | 1 | 0.42 | 1100 |
| CML 13 | AP | t(9;22) | 5 | 0.21 | 40 |
| CML 14 | BC, refractory | 7q−, inv3 | 21 | * | * |
| CML 3 | BC | t(9;22), (T315I) | * | * | |
| CML 5 | AP | t(9;22), (F359V) | * | * |
Samples span a wide range of AML subtypes by French-American-British and cytogenetic classification. CML samples encompass one patient with high Ph+ disease burden and samples from accelerated phase (AP) and blast crisis (BC) CML, some of which harbored BCR-ABL mutations. The CI values at 380nM MK1775, for which the strongest synergy was observed, are listed with the corresponding Ara-C concentrations. Samples are derived from BM.
Insufficient sample material was available for CalcuSyn analysis.
190nM MK1775.