Summary of the -fold sensitization results from siDDR experiments
| Gene . | Cell line . | |||
|---|---|---|---|---|
| TF-1 . | THP-1 . | HEL . | MDS-L . | |
| WEE1 | 4.0-17.5 | 5.6-25.0 | 3.7-8.3 | 1.1-2.1 |
| CHK1 | 1.3-13 | 1.9-29.0 | 1.0-1.5 | 1.0 |
| ATR | 1.9-4.3 | 1.5-2.4 | 1.2-3.4 | 1.4-2.1 |
| PKMYT | 2.9-3.8 | 2.0-7.0 | 1.0-1.4 | 1.0 |
| HGS | 2.0-6.6 | 1.2-1.5 | 1.4-1.5 | 1.2-1.6 |
| Gene . | Cell line . | |||
|---|---|---|---|---|
| TF-1 . | THP-1 . | HEL . | MDS-L . | |
| WEE1 | 4.0-17.5 | 5.6-25.0 | 3.7-8.3 | 1.1-2.1 |
| CHK1 | 1.3-13 | 1.9-29.0 | 1.0-1.5 | 1.0 |
| ATR | 1.9-4.3 | 1.5-2.4 | 1.2-3.4 | 1.4-2.1 |
| PKMYT | 2.9-3.8 | 2.0-7.0 | 1.0-1.4 | 1.0 |
| HGS | 2.0-6.6 | 1.2-1.5 | 1.4-1.5 | 1.2-1.6 |
Table shows the shift in Ara-C EC50 after silencing candidate sensitizing targets with 4 different siRNAs against each target. The range shows the weakest to strongest sensitization observed with different siRNA sequences. Silencing with 4 siRNAs per target was assessed in combination with 10 concentrations of Ara-C in quadruplicate wells from duplicate experiments. 1.0 = no sensitization. See supplemental Figure 3 for corresponding drug-dose–response curves/graphs.